Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (1): 222-230.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0611

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Genome-wide Association Study of Seed Glucosinolate Content in Brassica napus

ZHOU Hui-wen1(), WU Lan-hua1, HAN De-peng2, ZHENG Wei2, YU Pao-lan2, WU Yang1(), XIAO Xiao-jun2()   

  1. 1. Institute of Jiangxi Oil-tea Camellia, College of Pharmacy and Life Science, Jiujiang University, Jiujiang 332005
    2. Jiangxi Institute of Red Soil and Germplasm Resources, National Engineering and Technology Research Center for Red Soil Improvement, Jiangxi Key Laboratory of Red Soil Arable Land Conservation, Nanchang 330045
  • Received:2023-06-28 Online:2024-01-26 Published:2024-02-06
  • Contact: WU Yang, XIAO Xiao-jun E-mail:zhouhuiwen0320@126.com;yangwu15@126.com;xiao850908@163.com

Abstract:

【Objective】To explore SNPs and candidate genes significantly associated with seed glucosinolate content in rapeseed is helpful to improving rapeseed quality and cultivating high quality rapeseed varieties. 【Method】Total 300 oilseed rapeseed inbred lines grew in the experimental field of Jiangxi Agricultural University(JXAU)and Jiangxi Institute of Red Soil and Germplasm Resources(JXIRS), and the seed glucosinolate content(SGC)was analyzed after harvested. A total of 201 817 SNPs developed by SLAF-seq in all populations were used for genome-wide association with study(GWAS)of SGC. The 100 kb flanking regions on either side of significantly associated SNPs were used to identify candidate genes, and to perform functional annotations of these genes. 【Result】The SGC of these populations showed phenotypic variations under two environments. Based on the general linear model(GLM)the mixed linear model(MLM), 209 SNPs were detected to have significant association with SGC, and 41 SNPs on A05(1), A09(36)and C09(4)were detected by GLM and MLM under JXAU and JXIRS. The functional annotation of candidate genes showed that eight candidate genes participated in the glucosinolate biosynthetic process(GO:0019761), including regulating the glucosinolate synthesis related genes MYB28BnaC09g05290D and BnaC09g05300D), MYB34BnaA09g05480D), and the glucosinolate transporter 2 related genes(BnaA09g06180D and BnaA09g06190D). 【Conclusion】The SNPs significantly associated with SGC were detected under two environments by GLM and MLM, and the candidate genes were mined at the flanking of SNPs, which is conducive to resolving the genetic variation of SGC and providing a basis for the genetic improvement of new rapeseed varieties with low SGC.

Key words: Brassica napus, glucosinolate, GWAS, MYB transcription factor, glucosinolate transporter 2