Identification and Induced-expression Analysis of REVEILLE Family in Brassica napus L.

doi:10.13560/j.cnki.biotech.bull.1985.2023-1153

Abstract

Abstract:

【Objective】 REVEILLE family genes have important biological functions, but there are few studies on this family gene in Brassica napus L. This project was conducted to further understand the function of RVE family genes in B. napus and their relationship with abiotic stress. 【Method】 Bioinformatics methods were used to identify33, 16 and 16 RVE genes in B. napus, B. rapa and B. oleracea, and to analyze their phylogenetic development, chromosome localization, gene structure, physical and chemical properties, and promoter cis-acting elements. 【Result】 All RVE proteins were classified into two subfamilies. The 33 BnaRVE were located on 18 chromosomes of the A sub-genome and 15 chromosomes of the C sub-genome. Most members belonged to relatively stable hydrophobic proteins. Most of the Ka/Ks values were < 1, indicating that the family was subjected to strong purification selection. The gene structure varied greatly, and the number of introns ranged from 4 to 11. Collinearity analysis showed that there were large number of homologous genes in B. napus and Arabidopsis, B. rapa and B. oleracea. Most RVE family members of B. napus expressed RVE in the cotyledon and leaves, and the expression was the highest. BnaRVE promoter contained a large number of cis-acting elements related to hormone and biological stress. Through quantitative PCR analysis, the expressions of 4 BnaRVE genes were significantly upregulated under ABA and MeJA induction as well as low temperature stress. 【Conclusion】 Thirty-three members of the BnaRVE family were identified in the genome of B. napus. Different genes showed different expression patterns in different developmental stages and different tissues. Different genes have different expression patterns in response to various stresses. RVE family members have positive response function to ABA, MeJA and cold stress.

Key words: Brassica napus L., RVE genes family, bioinformatics analysis, adverse stress, phytohormone, expression analysis, expression profile

LIU Rong, TIAN Min-yu, LI Guang-ze, TAN Cheng-fang, RUAN Ying, LIU Chun-lin. Identification and Induced-expression Analysis of REVEILLE Family in Brassica napus L.[J]. Biotechnology Bulletin, 2024, 40(6): 161-171.

Figures/Tables 8

Table 1 Real-time fluorescent quantitative primers for RVE gene family expression analysis in B. napus

Gene name	Forword primer sequence（5'-3'）	Reverse primer sequence（5'-3'）
BnaRVE1d	TGTCTCCTGTTTCCTTTGCATCACC	AACTCTCTCTGTTTACGAGTCTCTCTCT
BnaRVE8b	TTCGACCTCCGGTATGGGAAG	GAATTACCTTGAAGCACCGGAGG
BnaRVE3c	GTGTGGCTCTTACAACTTCAAACGCAT	CACCCAATTCCTCTTTGATCACTGGAA
BnaRVE5b BnaActin2	TCTCCACTTATTCGATCGAGATTGGAAG GTCTTCTCTGCTCTTCTCA	GTTCGTTAGCACCGCTCTTCTG CCATTCCAGTTCCATTGTC

Fig. 1 Phylogenetic analysis of RVE family in A. thaliana, B. napus, B. rapa and B. oleracea

Fig. 2 Chromosomal location of the RVE family gene in B. napus

Fig. 3 Conserved motif, domain and gene structure of RVE family members in B. napus

Fig. 4 Genomic distribution of cis-acting elements binding to promoter region of RVE family members in B. napus（A）and the top 10 CRE（B）in genome

Fig. 5 Intra-specic collinearity analysis of B. napus RVE family（A）and collinearity analysis of RVE family genes among A. thaliana, B. napus, B. rapa and B. oleracea（B-D）

Fig. 6 Gene expression analysis of RVE family in B. napus

Fig. 7 RT-qPCR expression analysis of BnaRVE under different stress treatments A: Expression of BnaRVE in MeJA processing.B: Expression of BnaRVE in ABA processing. C: Expression of BnaRVE in cold treatment

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