Identification of the NAC Transcription Factors in Nymphaea colorata and Their Expression Analysis

doi:10.13560/j.cnki.biotech.bull.1985.2024-0642

Abstract

Abstract:

Objective This study aims to identify the NAC members in waterlily (Nymphaea colorata), and to investigate their evolutionary history and expressions under different tissues and ABA treatment. The current study not only could provide a key theoretical basis on the molecular evolution of the waterlily NAC family, but also could obtain some important candidate genes about regulating floral organ morphogenesis and stress response in waterlily. Method The whole-genome methods were applied to analyze the conserved motifs, gene structures, phylogenetic evolutions, chromosomal locations, gene duplication events, and cis-regulatory elements in the promoter regions of the waterlily NAC family. The expression levels of the waterlily NAC members under different tissues and ABA treatment were performed through the transcriptome and RT-qPCR. Result In this study, 64 NAC members screened from waterlily were divided into 15 subfamilies. The NcNAC members were unevenly distributed on the waterlily's 14 chromosomes, with most members located on chromosomes 1, 2, 9, and 11. Segmental duplication events were prevalent in the expansion of the NcNAC members. Meanwhile, NcNAC11, NcNAC43, NcNAC36 and NcNAC52 were highly expressed in floral organs. Thus, NcNAC11, NcNAC43, NcNAC36 and NcNAC52 may regulate the flower growth and development in waterlily. In addition, numerous stress-responsive regulatory elements were found in the promoter regions of the NcNAC members. NcNAC07, NcNAC08, NcNAC34, and NcNAC43 had the most ABA responsive elements in their promoter regions, and these NcNAC members responded to ABA stress, indicating that NcNAC07, NcNAC08, NcNAC34, and NcNAC43 might be related to stress resistance. Conclusion There are 64 NcNAC members in waterlily, and these NcNACs could be classified into 15 subfamilies. NcNAC11, NcNAC43, NcNAC36 and NcNAC52 have the highest expression in the floral-related tissues, and NcNAC07, NcNAC08, NcNAC34, and NcNAC43 may respond to ABA stress.

Key words: Nymphaea colorata, NAC family, transcription factor, molecular evolution, expression analysis

QIAN Zheng-yi, WU Shao-fang, CAO Shu-yi, SONG Ya-xin, PAN Xin-feng, LI Zhao-wei, FAN Kai. Identification of the NAC Transcription Factors in Nymphaea colorata and Their Expression Analysis[J]. Biotechnology Bulletin, 2025, 41(2): 234-247.

Figures/Tables 10

Table 1 Primer sequences of RT-qPCR in this study

基因名称Gene name	正向引物Forward primer （5′-3′）	反向引物Reverse primer （5′-3′）
NcUbi	CCTGTATTGCCTGATG	GACGAGATGGTGGAGT
NcNAC11	CGGTGTTGACAAGACGGAGA	CGTCCTCTTCGATGGTAGGC
NcNAC07	CAGGAGAGGGAAGCACAAGG	GTTGCCGAAGACAAAGCCAG
NcNAC08	GCTTCAGGTTCCATCCGACA	TTGGCACTGAAATTGGCTGC
NcNAC33	GGATGAGTACCGTCTCGCAG	CGTCCAAGTCGTCCAGTGAA
NcNAC34	TCCGGTTACTGGAAAGCCAC	ACTCGTGCATGATCCAGTCC
NcNAC36	CCTACAGCTGCACCCTTCAA	GGTGATCTATCAGTGCCGGG
NcNAC43	GGTGACAGCAGGAACGAAGA	CGCTGGTGATGATGCTTTCG
NcNAC52	ACTCTACCTGCATCGGTCCT	GGGATGAATGTGAAGGGGCA
NcNAC56	ATTTGCCTGGTGATGGGGAG	ACAACTTGGTTGCACCTGGA

Fig. 1 Phylogenetic analysis of the NAC family in A. thaliana and N. colorata

Fig. 2 Phylogenetic analysis (A) and distribution of conserved motif (B) and gene structure (C) in the NAC family of N. colorata

Fig. 3 Chromosomal distribution of NAC members in N. colorata

Fig. 4 Syntenic analysis and Ks distribution of the duplicated NAC members in N. colorataA: Syntenic analysis of the duplicated NcNACs. B: Subfamily distribution of the duplicated NcNACs. C: Ks distribution of the duplicated NcNACs, and the arrow points to peak value of Ks

Fig. 5 Stress-responsive regulatory elements in the promoter regions of NcNAC members

Fig. 6 Expression profiles of NcNAC members in different tissues through the transcriptome data

Fig. 7 Expression profiles of NcNAC members in different tissues by RT-qPCR methodDifferent letters indicate the significant differences among different treatments at the 0.05 level. The same below

Fig. 8 Relationship network of the NcNAC members in N. colorata

Fig. 9 Expressions of the NcNAC members in N. colorata under ABA stress

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