Application and Research Progress of Gene Editing Technology in Bovine

doi:10.13560/j.cnki.biotech.bull.1985.2024-1083

Abstract

Abstract:

Gene editing refers to the process of modifying specific targets in an organism’s genome through gene editing techniques, achieving efficient and precise gene knockout, insertion, replacement, and other processes. Modern gene editing techniques include zinc finger nucleases (ZFNs), transcription activator like effector nucleases (TALENs), clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9 (CRISPR/Cas9), and self-cleaving gene targeting technology in combination with programmable nucleases (SEGCPN). This paper reviews the breakthrough research progress of gene-edited cattle, including the famous gene-edited hornless cattle Buri, Spotigy and the US Food and Drug Administration (FDA) safety certified PRLR-SLICK, and summarizes the application of gene-edited cow in production, medicine and environment. The aim is to summarize the current application and research progress of gene editing technology in domestic cattle, in order to accelerate the industrialization process of gene editing technology in China and expand the application range of gene editing cattle.

Key words: gene-edited cattle, ZFNs, TALENs, CRISPR/Cas9, SEGCPN

YU Yong-xia, DU Zai-hui, ZHU Long-jiao, XU Wen-tao. Application and Research Progress of Gene Editing Technology in Bovine[J]. Biotechnology Bulletin, 2025, 41(8): 34-41.

Figures/Tables 1

References 48

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