Fig. 1 Quality control of mass spectrometry dataA: Total protein identification by mass spectrometry. B: Charge and length distribution of specific peptides. C: Peptide number distribution of quantified proteins. D: Relative molecular weight distribution of quantified proteins

Fig. 2 Sample reproducibility analysisA: PCC analysis heatmap. B: PCA Principal component analysis. C:RSD Analysis violin plot. D: RSD analysis boxplot

Fig. 3 Differential protein screeningA: Volcano plot. B: Clustering heatmap

Fig. 4 GO functional enrichment analysis of differentially expressed proteinsA: GO enrichment bubble plot of upregulated differentially expressed proteins. B: GO enrichment bubble plot of downregulated differentially expressed proteins

Fig. 5 KEGG functional enrichment analysis of differentially expressed proteinsA: KEGG enrichment bar plot of upregulated differentially expressed proteins. B: KEGG enrichment bar plot of downregulated differentially expressed protein

Fig. 6 Domain enrichment analysis of differentially expressed proteins

Fig. 7 Subcellular localization analysis

Fig. 8 Protein-protein interaction network analysis of differentially expressed proteinsEllipses and squares indicate differentially expressed proteins in the branched-chain amino acid synthesis pathway that interact with proteins, where ellipses interact with the AFLA_000930 protein; triangles indicate differentially expressed proteins in the aflatoxin synthesis pathway that interact with proteins; diamonds indicate connecting node proteins. Red indicates upregulated differentially expressed proteins; blue indicates downregulated differentially expressed proteins

Fig. 9 Expression determination of aflatoxin biosynthesis-related genes

Fig. 10 Analysis of intracellular metabolic reprogramming× indicates metabolic pathways affected by AHAS functional deficiency; red indicates upregulated metabolic pathways; blue indicates downregulated metabolic pathways