Microbial Diversity of the Crude Oil and Characteristics of a Hydrocarbon-degrading Bacterium

Abstract

Abstract: The biodegradation bacterium system was obtained through adding nutrition, and the IR Technology, GC analysis and UVmethond were used. The analyse showed high degradation ability for crude oil. The diversity of bacterial communities in crude oil was researchedusing 16S rDNA library approach. A high efficiency bacterium was obtained from the biodegradation bacterium system, which was identified asBacillus pumilus by automated cellular fatty acid identification system and phylogenetic analyses based on the 16S rDNA sequence. GC analysisshowed that the low-carbon chain alkanes and high-carbon-chain alkanes as well as aromatic hydrocarbons can be degraded.

Key words: Crude oil , IR technology , 16S rDNA , Biodegradation , Gas chromatography

Wang Zhonghua, Liang Jinger, Yang Jianqiang, Zhou Jun, Li Chenghua, Li Taiwu. Microbial Diversity of the Crude Oil and Characteristics of a Hydrocarbon-degrading Bacterium[J]. Biotechnology Bulletin, 2013, 0(1): 178-185.

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