Screening and Characteristics of a Broad Spectrum Fungus Degrading Polycyclic-aromatic Hydrocarbons：Aspergillus flavus AD-X-1

doi:10.13560/j.cnki.biotech.bull.1985.2018-0125

Abstract

Abstract: This study is aimed to screen a broad spectrum strain degrading polycyclic-aromatic hydrocarbons（PAHs）and investigate its degradation characteristics for providing strain supply and technical support in the management of combined pollution. An efficient PAHs-degradation fungus was screened with PAHs as the sole carbon and energy source and identified and designated as Aspergillus flavus AD-X-1. The experiment convinced that the degradation was predominant in the removal of PAHs by AD-X-1,while mycelium adsorption also played a part role. The degradation condition was optimized using anthracene as the substrate,and the removal rate of anthracene reached 88% in 72 h by AD-X-1 under the condition of 50 mg/L anthracene,temperature 35℃,rotator speed 170 r/min and pH 7. Further AD-X-1 was found to have promising salt-tolerance,and the removal rate of anthracene still remained at 50% when the salinity increased to 9%. Moreover,AD-X-1tolerated higher concentration of heavy metal ions（Cr³⁺,Cu²⁺,and Pb²⁺）. In addition to anthracene and phenanthrene,the removal rate of tetracyclic pyrene,benzo[a]anthracene,and dibenzo[a,h]anthracene by AD-X-1 were 71%,68%,and 63% respectively. Conclusively,AD-X-1 has broad spectrum ability of removing PAHs,and concurrently it has the excellent resistances to salt and heavy metals.

Key words: combined pollution, biodegradation, heavy metals, salt tolerance

TIAN Jing, XU Xiao-lin, KANG Yan-shun, TANG Wei-hua, LIU Si-qi. Screening and Characteristics of a Broad Spectrum Fungus Degrading Polycyclic-aromatic Hydrocarbons：Aspergillus flavus AD-X-1[J]. Biotechnology Bulletin, 2018, 34(8): 115-122.

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