Determination Methods for Lipase Activity and Its Application in the Screening of Microbial Lipase

Abstract

Abstract: Four common lipase activity determination methods including Rhodamine B plate, olive oil emulsification, ρ-NPP for lipasehydrolytic activity and ρ-NPP for lipase synthetic activity were compared. The result showed that the Rhodamine B plate method was suitable forthe qualitative and preliminary quantitative estimate of lipase activity and the other three methods were suitable for the quantitative determinationof lipase activity but only the ρ-NPP method has a good reproducibility. Moreover, there is no relationship between the hydrolytic activity andsynthetic activity of lipase. So lipase activity determination method should be chosen in screening the lipase-produced microbial. Namely,hydrolytic activity determination methods should be chosen in screening the hydrolytic lipase-produced microbial, otherwise, ester synthetaseactivity assay should be used. Based on the principle, desired lipase-produced microbial were screened.

Key words: Lipase , Hydrolytic activity , Synthetic activity , Determination methods , Screening

Wang Huan, He Laping, Zhou Huanjing, Zhang Yiming, Li Cuiqin, Tao Han. Determination Methods for Lipase Activity and Its Application in the Screening of Microbial Lipase[J]. Biotechnology Bulletin, 2013, 0(1): 203-208.

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