Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (3): 129-133.

• Research report • Previous Articles     Next Articles

Expression and Antibody Preparation of Plutella xylostella Granulovirus PP31

Wang Simin, Zhang Hongjie, Li Lulin   

  1. Hubei Key Laboratory of Genetic Regulation and Integrative Biology,College of Life Sciences,Central China Normal University,Wuhan 430079)
  • Received:2012-07-03 Revised:2013-03-21 Online:2013-03-20 Published:2013-03-21

Abstract: Baculovirus PP31 is a phosphoprotein that could combine with DNA. The pp31 gene presents in all of the genomes of lepidopteran baculoviruses sequenced to date. In this study, Plutella xylostella pp31 gene was PCR-amplified and cloned into an expression vector pET28a, and transformed into the E. coli BL21(DE3). In the transformed bacterial cells, induced by IPTG, a His-tagged PP31 protein with a size of 29 kD was expressed, which was purified using ProbondTM resin. Polyclonal antibodies against PlxyGV PP31 were prepared by immunizing a New Zealand rabbit with the purified his-tagged PP31. Western blot analysis showed that the antiserum had high titer and specificity and could be used in study of PP31.

Key words: Plutella xylostella granulovirus(PlxyGV), PP31, Prokaryotic expression, Polyclonal antibody