Tetra-primer Amplification Refractory Mutation System PCR and Its Application in Fauna and Flora Genetics and Breeding Research

doi:10.13560/j.cnki.biotech.bull.1985.2019-0575

Abstract

Abstract:

Tetra-primer amplification refractory mutation system PCR（tetra-primer ARMS PCR）is a technique of typing SNP developed on the basis of common PCR. This technique combines the advantages of amplification refractory mutation system（ARMS）and four-primer PCR（tetra-primer PCR）,is an improvement of allele specific PCR. It has the characteristics of simple operation,rapid typing and low cost and has been applied more and more widely in the field of life sciences especially in the field of breeding. In this paper,the principle and advantages of tetra-primer ARMS PCR technique,detection methods of results and improvement methods of reaction system are introduced. In addition,the applications of this technique in genetics and breeding researches are reviewed.

Key words: tetra-primer amplification refractory mutation system, single nucleotide polymorphism, genetics and breeding

YU Jun-jian, CHI Mei-li, JIA Yong-yi, LIU Shi-li, ZHU Jun-quan, GU Zhi-min. Tetra-primer Amplification Refractory Mutation System PCR and Its Application in Fauna and Flora Genetics and Breeding Research[J]. Biotechnology Bulletin, 2020, 36(5): 32-38.

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