Abstract: It was to optimize the conditions of AChRs expressed on Xenopus laevis oocytes, including the oocytes’ digestion time, frequency and the amount of collagenase. Results showed that when using two-step digestion method and 0.5 mg/mL concentration of collagenase, the isolated Xenopus laevis oocytes was beneficial to express AChRs. The conditions of receptor expression on the oocytes were also optimized. The optimized expression methods are significant to set up the expression system of AChRs on Xenopus laevis oocytes, which are the basis for screening new drugs to target AChRs with this experimental model.

Key words: Xenopus laevis oocytes, Acetylcholine receptor, Expression, Optimization