Screening and Sequence Analysis of Cashmere Goat DBY

Abstract

Abstract: Cashmere goat is a unique biological resource in China. After a long period of natural selection and artificial breeding, Cashmere goat has best fiber quality and higher cashmere yields at present in the world. Therefore, the high reproductive rate of Cashmere goat is essential for developing economy. In this experiment, 240 bp gene fragment was obtained, though screening from Cashmere goat BAC library, cloning, and identifying DBY gene controlling spermatogenesis on the Y chromosome by PCR. Then the sequence of Cashmere goat was compared with other species from GenBank. The results showed Cashmere goat has homology of 96% with Ovis aries, homology of 93% with Cervus eld and Bubalus bubalis. The homology is up to 92% and 91% with Axis porcinus, Bos indicus and Bos teurus, respectively, no homology with the human, chimpanzee and mouse. The sequence fragment encodes 52 amino acids. The clustering consequence of species NJ consistent with the taxonomic status.

Key words: Cashmere goat, DBY, BAC, Sequence analysis

Xiao Hongmei,Xiao Xu,Liu Zhihong,Li Jinquan. Screening and Sequence Analysis of Cashmere Goat DBY [J]. Biotechnology Bulletin, 2013, 0(7): 89-93.

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