Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (8): 43-50.

• Research Report • Previous Articles     Next Articles

Comparison on Stress-resistent Phenotypes Between Arabidopsis pumila and Arabidopsis thaliana and Expression Analysis

Zhang Tingting, Zhou Yaping, Zeng Youling   

  1. (Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Urumqi 830046)
  • Received:2013-03-13 Revised:2013-08-11 Online:2013-08-11 Published:2013-09-02

Abstract: In this study, the data of stress-resistent phenotypes were compared between Arabidopsis pumila and Arabidopsis thaliana and the expression of some stress-responsed genes was examined by Real-time PCR in Arabidopsis pumila subjected to the salt, mimic drought, ABA and cold stress. Some results were as followed:(1)The water-retention capacity of Arabidopsis pumila was stronger than Arabidopsis thaliana under the same condition with the determination of relative water content between two species.(2)Arabidopsis pumila showed more stressed resistence than Arabidopsis thaliana as model plant under salt, mimic drought, heat and ABA stress by the observation on the growth phenotypes and the statistical analysis of relative root length between two species. Arabidopsis pumila didn't present a better growth phenotype than Arabidopsis thaliana after a cold stress treatment, then a normal growth condition.(3)The expressions of some stress-responsed genes in Arabidopsis pumila, such as RD22, RD29A and ADH, had significant increases in most case under the treatments of salt, mimic drought, cold and ABA.

Key words: Arabidopsis pumila, Arabidopsis thaliana, Comparison on stress-resistent phenotypes, Stress-responsed genes, Expres-sion analysis