Response to NaCl and ABA in Arabidopsis thaliana of the Double Silent Gene VHA-c2&c4

doi:10.13560/j.cnki.biotech.bull.1985.2020-0098

Abstract

Abstract: For studying the functions of vacuole H⁺- ATPase subunit c gene（VHA-c2 and VHA-c4）in plant growth and response to abiotic stress,we constructed an RNAi expression vector of VHA-c2&c4 and then transfected it into wild-type Arabidopsis thaliana plants by the floral dip method. Transgenic homozygotes were screened and identified by kanamycin,the expression levels of VHA-c2&c4 were analyzed by semi-quantitative RT-PCR,and the treatments of NaCl and ABA on them were conducted. The results showed that 7 T₂lines of double silent gene homozygous were obtained,and their mRNA expression levels of the two genes were lower than the wild type. Three lines with high silencing efficiency were selected for NaCl and ABA treatment. The relative elongation of primary roots and seed germination rate were both lower than that of the control group under NaCl treatment,indicating that the lines of VHA-c2&c4 gene silencing were sensitive to NaCl stress. The cotyledon expansion and the relative elongation of the primary root were both higher than the control under ABA treatment,indicating that the lines of the VHA-c2&c4 gene silencing were not sensitive to ABA inhibition. The results of NMT experiment showed that ABA promoted the H⁺ inflow ability of double-silenced gene homozygote lines. We hypothesized that the transcriptional levels of the genes have an impact on plant responses to salt stress and ABA-mediated signaling pathways.

Key words: Arabidopsis thaliana, vacuolar-type H⁺-ATPase, RNA interference, NaCl, ABA

SU Jie, GUO Rong-qi, GAO Yang, YU Xiu-min, LI Guo-jing, WANG Rui-gang. Response to NaCl and ABA in Arabidopsis thaliana of the Double Silent Gene VHA-c2&c4[J]. Biotechnology Bulletin, 2020, 36(7): 48-54.

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