哈茨木霉SKD-ZX-1的鉴定、发酵及其生防效果

doi:10.13560/j.cnki.biotech.bull.1985.2019-0586

摘要/Abstract

摘要： 哈茨木霉作为一类重要的生防菌,对蔬菜病害致病菌具有拮抗作用。为确定分离的哈茨木霉SKD-ZX-1的生防效果,利用气质联用技术对菌株的发酵液进行测定和分析,利用宏基因组测序方法分析该菌株对番茄根腐烂病土壤中的细菌菌群的影响,并且采用平板对峙培养法对该菌株与链格孢菌和茄链格孢菌进行了拮抗实验。结果显示,哈茨木霉SKD-ZX-1发酵液中有邻苯二甲酸二异丁酯、邻苯二甲酸二丁酯和邻苯二甲酸二（2-乙基己基）酯等抑菌成分,并且其发酵液增大了土壤中细菌菌群的种类,与对照组相比,实验组肠杆菌属提高了46%,而假单胞菌属和梭菌属降低了5.28%和36.1%。该哈茨木霉对两种病原菌的抑制率均达到100%,拮抗系数达Ⅰ级。研究表明哈茨木霉SKD-ZX-1对链格孢菌和茄链格孢菌具有显著的抑制作用,其发酵液具有一定的抑菌效果。

关键词: 哈茨木霉, 拮抗, 宏基因, GC-MS

Abstract: Trichoderma harzianum is an important biocontrol bacterium and has an antagonistic effect on pathogenic bacteria of vegetable diseases. In order to determine the biocontrol effect of the isolated T. harzianum SKD-ZX-1,the fermentation broth of the strain was determined and analyzed by GC-MS,and the effect of this strain on the bacterial flora in tomato root rot soil was analyzed by metagenomic sequencing. Further the antagonistic experiment of the strain against Alternaria spp. and Alternaria solani were carried out by plate-to-sputum culture method. The results showed that the fermentation liquid of T. harzianum SKD-ZX-1 contained multiple antibacterial ingredients,namely diisobutyl phthalate,dibutyl phthalate,and di（2-ethylhexyl）phthalate,and increased the types of bacterial flora in the soil. Moreover,Enterobacter increased by 46% in the experimental group compared with the control group,while the genus Pseudomonas and Clostridium decreased by 5.28% and 36.1%,respectively. The inhibition rates of T. harzianum against the two pathogens was 100%,and the inhibition coefficient reached Ⅰ. The present study demonstrates that T. harzianum SKD-ZX-1 has significant inhibitory effect on Alternaria alternata and Alternaria solani,and its fermentation broth has certain antibacterial effect.

Key words: Trichoderma harzianum, antagonism, macrogenomics, GC-MS

陆洪省, 张雪, 高宇婷, 孙珮铭, 邱萌萌. 哈茨木霉SKD-ZX-1的鉴定、发酵及其生防效果[J]. 生物技术通报, 2019, 35(11): 132-140.

LU Hong-sheng ZHANG Xue GAO Yu-ting SUN Pei-ming QIU Meng-meng. Identification,Fermentation and Biocontrol Efficiency of Trichoderma harzianum SKD-ZX-1[J]. Biotechnology Bulletin, 2019, 35(11): 132-140.

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