生物技术通报 ›› 2021, Vol. 37 ›› Issue (4): 56-69.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0702

• 研究报告 • 上一篇    下一篇

川芎非根际、根际及根茎内生真菌群落差异分析

张秫华1(), 方千1, 贾红梅1, 韩桂琪1,2, 严铸云1, 何冬梅1()   

  1. 1.成都中医药大学药学院 中药资源系统研究与开发利用国家重点实验室培育基地,成都 611137
    2.成都中医药大学医学技术学院,成都 611137
  • 收稿日期:2020-06-08 出版日期:2021-04-26 发布日期:2021-05-13
  • 作者简介:张秫华,女,硕士研究生,研究方向:药用植物微生态;E-mail:1729088613@qq.com
  • 基金资助:
    国家自然科学基金项目(81673553);四川省科技厅创新苗子工程重点项目(2017RZ0054);中央本级重大增减支项目(2060302);成中医杏林人才计划(030041141)

Difference Analysis of Fungal Community Among Bulk Soil,Rhizosphere and Rhizomes of Ligusticum chuanxiong Hort.

ZHANG Shu-hua1(), FANG Qian1, JIA Hong-mei1, HAN Gui-qi1,2, YAN Zhu-yun1, HE Dong-mei1()   

  1. 1. College Pharmacy of Chengdu University of Traditional Chinese Medicine,Key Laboratory of the Ministry of Education for the Standardization of Traditional Chinese Medicine,Chengdu 611137
    2. Medical Technology College of Chengdu University of Traditional Chinese Medicine,Chengdu 611137
  • Received:2020-06-08 Published:2021-04-26 Online:2021-05-13

摘要:

获知并比较川芎非根际、根际与川芎根茎内生真菌的群落结构特征及其差异。利用高通量测序技术分析川芎非根际土(S)、根际土(R)及川芎根茎(P)的真菌群落结构及差异;采用FUNGuild分析真菌群落功能。川芎非根际土、根际土及川芎根茎中皆含有丰富的真菌类群,真菌群落均匀度和多样性表现为S>R>P,丰富度表现为R>S>P。在不同分类水平下,3组样本皆具各自的优势菌群。川芎非根际与根茎的真菌群落差异最大,根际土与植物次之,土壤之间差异最小。川芎非根际、根际及根茎内生真菌皆具有产地差异,且大小为S>R>P,环境因子对非根际及根际菌群影响效应为S>R。川芎非根际土真菌以腐生型为主,其次为病原菌型,根际土真菌以病原菌型为主,腐生型次之,川芎内生菌则以病原菌型真菌为主。从非根际土到根际土再到川芎根茎内部,真菌菌群结构特征及菌群生态功能呈连续的渐次性变化,证明川芎在栽培过程中,从产地土壤中获得部分物种并发生了连续积累或消减,形成特定的内生菌群,是其微生态群落结构构建的重要策略,这为理解中药道地性品质形成提供了科学参考。

关键词: 川芎, 高通量测序, 群落多样性, 根际, 内生真菌

Abstract:

It is aimed to obtain and compare the characteristics of fungal communities in bulk soil,rhizosphere and endophytic fungi of rhizome of Ligusticum chuanxiong Hort. High-throughput sequencing technology was used to analyze the structures and differences of fungal communities in bulk soil(S),rhizosphere(R)and endophytic fungi(P)of the rhizome of L. chuanxiong Hort. FUNGuild was used to analyze the functions of fungal communities. There were high abundant fungal communities in the bulk soil,rhizosphere and endophytic fungi of the rhizome of L. chuanxiong Hort. The evenness and diversity of the fungal community showed as S>R>P,while the richness showed as R>S>P. Specific dominated fungal flora varied in the three groups at different taxonomic levels. Meanwhile,the fungal communities were mainly affected by the sample type,with the largest differences between bulk soil and rhizome,followed by that between rhizosphere and plants,and the least among the soils. Differences among origin areas were observed in the three groups separately,and ranked as S>R>P. Besides,the effect of environmental factors on the communities of the bulk soil and rhizosphere was S>R. The fungal community in the bulk soil was dominated by saprotroph,followed by pathotroph;while the rhizosphere soil was dominated by pathotroph,followed by saprotroph;and the endophytic fungi was dominated by pathotroph. The fungal structural characteristics and ecological functions presented continuous and gradual changes in the bulk soil to the rhizosphere soil and then to the rhizome of L. chuanxiong Hort.,which proved that the plants obtained special fungi from the soil during the cultivation process,continuous accumulation and reduction of these fungi occurred,and then special endophytic fungal community was formed. This way may be an important strategy for the construction of its microecological community structure and provide a scientific reference for understanding the formation of the authentic quality of Chinese medicine.

Key words: Ligusticum chuanxiong Hort., high-throughput sequencing, community diversity, rhizosphere, endophytic fungi