一株异养硝化细菌的分离鉴定及紫外诱变育种

生物技术通报 ›› 2013, Vol. 0 ›› Issue (12): 184-188.

一株异养硝化细菌的分离鉴定及紫外诱变育种

郑丽侠张大伟

齐鲁工业大学食品与生物工程学院山东省微生物工程重点实验室，济南 250353

收稿日期:2013-07-05 出版日期:2013-12-20 发布日期:2013-12-20
作者简介:郑丽侠，女，硕士研究生，研究方向：废水生物处理； E-mail：zhenglixiahappy@163.com

Isolation and Identification of a Heterotrophic Nitrifying Bacterium and Its Mutation Breeding by UV-irradiation

Zheng Lixia, Zhang Dawei

Key laboratory of Microbial Engineering of Shandong Province，Food and Bioengineering Department of Qilu
University of Technology， Ji’ nan 250353

Received:2013-07-05 Published:2013-12-20 Online:2013-12-20

摘要/Abstract

摘要： 以活性污泥为主要来源，筛选得到一株具有硝化作用的异养型细菌N18，对其16S rDNA进行测序分析，确定N18为假单胞菌属（Pseudomonas sp.）。以70%--80%致死率为紫外诱变条件，经高浓度氨氮（15 g/L）培养基初筛得到8株突变株，以脱氮效率为复筛指标，得到一株氨氮去除率最高的突变株NF34。经过72 h培养，突变株NF34氨氮去除率达到85.06%，比诱变前菌株N18提高了11.99%，初始氨氮浓度分别为100、200和500 mg/L时，脱氮率分别提高了6.14%、11.49%、10.75%，在此过程中，亚硝态氮积累量较小，证明诱变也是获取高活性硝化细菌的有效手段。

关键词: 生物脱氮, 异养硝化作用, 假单胞菌, 紫外诱变

Abstract: A strain of heterotrophic nitrification bacteria named N18 was isolated from activated sludge of fermentation wastewater treatment using heterotrophic nitrification medium, and identified as Pseudomonas sp. according to the analysis of its16S rDNA gene. By UV-irradiation and 8 mutants was selected by high concentration of (NH4 )2 SO4 medium, and a mutant NF34 with highest rate of NH4 +-N removal was obtained. Studied on its function of heterotrophic nitrification, the result showed that the rate of NH4 +-N removal of strain NF34 was 11.99% higher than the original strain N18, the nitrification rate of different initial ammonia concentration 100, 200, 500 mg/L were 6.14%, 11.49%, 10.75% higher than the original strain, respectively. Little nitrite accumulated during nitrifying process. It proved that UV-mutation is an effective means to obtain nitrifying bacteria with highly nitrification activity.

Key words: Biological nitrogen removal, Heterotrophic nitrification, Pseudomonas sp. , UV-irradiation

郑丽侠, 张大伟. 一株异养硝化细菌的分离鉴定及紫外诱变育种[J]. 生物技术通报, 2013, 0(12): 184-188.

Zheng Lixia, Zhang Dawei. Isolation and Identification of a Heterotrophic Nitrifying Bacterium and Its Mutation Breeding by UV-irradiation[J]. Biotechnology Bulletin, 2013, 0(12): 184-188.

参考文献

[1] Taylor SM, He Y, et al. Heterotrophic ammonium removal character-istics of an aerobic heterotrophic nitrifying-denitrifying bacterium. Journal of Environmental Sciences, 2009, 21（10）：1336-1341.
[2] Papen H, Berg RV. A most probable number method（MPN）for the estimation of cell numbers of heterotrophic nitrifying bacteria in soil[J]. Plant and Soil, 1998, 199：123-130.
[3] Schmidt I, Sliekers O, Schmid M, et al. New concepts of microbial treatment process for the nitrogen removal in wastewater[J]. FEMS Microbiology Reviews, 2003, 27：481-492.
[4] 张彤, 庞金钊, 杨宗政, 等. 异养硝化菌的脱氮研究[J]. 水科学与工程技术, 2006, 6：41-42.
[5] Stephenson T, Judd S, Jefferson B, Brindle K. Membrane bioreactors for wastewater treatment[M]. London：IWA Publishing, 2000.
[6] Islam A, et al. Heterotrophic and autotrophic nitrification in two acid pasture soils[J]. Soil Biology & Biochemistry, 2007, 39（4）：972-975.
[7] Burton J, Chen C, Xu Z, Ghadiri H. Gross nitrogen transformations in adjacent native and plantation forests of subtropical Australia[J]. Soil biology & biochemistry, 2007 39（2）：426-433.
[8] 陈威, 王振宇, 赵更峰, 等. 异养硝化微球菌的分离及性能评价[J]. 生态学杂志, 2010, 29（8）：1629-1633.
[9] 刘芳芳, 周德平, 等. 养殖废水中异养硝化细菌的分离、筛选和鉴定[J]. 农业环境科学学报, 2010, 29（11）：2232-2237.
[10] 钟君伟, 鄢庆枇, 纪荣兴, 等. 高效好氧反硝化菌的紫外诱变育种[J]. 集美大学学报, 2011, 16（1）：14-19.
[11] 吕聪, 马玖彤, 刘懿, 等. 紫外诱变选育优势好氧反硝化菌株[J]. 环境科学学报, 2008, 28（10）：1976-1979.
[12] 辛玉峰, 等. 一株异养硝化-反硝化不动杆菌的分离鉴定及脱氮活性[J]. 微生物学报, 2011, 51（12）：1646-1654.
[13] 魏巍, 黄廷林, 等. 1株贫营养好氧反硝化菌的分离鉴定及其脱氮特性[J]. 生态环境学报, 2010, 19（9）：2166-2171.
[14] 国家环境保护总局. 水和废水监测分析方法[M]. 第4版. 北京：中国环境科学出版社, 2002.
[15] 赵云霞, 赵宗升, 陈智均. 谈废水生物脱氮过程中的几个问题[J]. 山西建筑, 2004, 30（17）：108-109.
[16] 刘晶晶, 等. 一株异养硝化-好氧反硝化菌的脱氮性能研究[J]. 环境科学研究, 2008, 21（3）：121-125.
[17] 廖小红, 汪苹, 等. 蜡状芽孢杆菌WXZ-8的异养硝化/好氧反硝化性能研究[J]. 环境污染与防治, 2009, 31（7）：17-24.