Research Progress of Gene Silencing by RNA Interfering Technology in Mammal

Abstract

Abstract: Variation of metabolic pathways result from RNA interference and related RNA silencing pathways have revolutionized our understanding of gene regulation. Gene silencing has been used as a research tool to control the expression of specific genes in numerous experimental organisms. Importing the different cells with specific siRNA need different transfection reagent to achieve maximum transfection efficiency, and will not produce larger cell toxicity. In recent years, new nano-material such as carbon nanotubes exert interference effect expanding us the traditional liposomes and virus’ understanding. The rapid progress in the field of RNA interference was summarized, and then compare different RNA interference experiments used several different carriers. Further, the perspectives of future research on RNA interference mediated from traditional liposomes to virus and polymer nano-material in disease treatment and clinic diagnose were proposed.

Key words: RNA interference , Small interfering RNA, Gene silencing , Transfection , Carbon nanotubes

Cao Tengwei,Gu Lingyun,Huang He,Gao Zhen,Li Mingfang. Research Progress of Gene Silencing by RNA Interfering Technology in Mammal[J]. Biotechnology Bulletin, 2014, 0(11): 24-31.

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