Biotechnology Bulletin ›› 2017, Vol. 33 ›› Issue (12): 156-161.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0556

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Role of Long Non-coding RNA AK043773 in Adipocyte Differentiation

ZHANG Juan-juan, QIAO Ling, ZHU En-dong   

  1. Key Laboratory of Hormones and DevelopmentMinistry of Health,Tianjin Key Laboratory of Metabolic Diseases,Metabolic Diseases Hospital & Institute of Endocrinology,Tianjin Medical University,Tianjin 300070
  • Received:2017-07-04 Online:2017-12-25 Published:2017-12-21

Abstract: This work is to investigate the expression changes and regulating role of long non-coding RNA(lncRNA)AK043773 in adipocyte differentiation. First,pre-adipocyte cells in mice were induced for adipogenesis. Then,the expressions of adipogenic differentiation regulating gene(KLF7)and AK043773 were measured by real-time quantitative PCR(RT-qPCR). Next,the overexpression vector for AK043773 was constructed for detecting its effects on adipogenic differentiation. According to search in UCSC database,the locus of AK043773 was in the 3'-end downstream of KLF7’s exon 4. The expressions of KLF7 and AK043773 were down-regulated coordinately in adipogenesis-induced and differentiated bone marrow stromal cell line ST2 and pre-adipocyte cells 3T3-L1. Furthermore,the overexpression vector pCDNA3.1-AK043773 was constructed and transfected into ST2 cells,which significantly enhanced the expression of AK043773,but inhibited the adipogenesis in ST2 cells by oil red O staining and OD520nm absorbance test after the induction of adipocyte differentiation. All of results indicate that AK043773 and KLF7 coordinately down-regulate in adipocyte differentiation,and increasing the expression of AK043773 remarkably suppresses the differentiation of adipocyte.

Key words: lncRNA, AK043773, adipocyte differentiation, KLF7