Identification,Fermentation and Biocontrol Efficiency of Trichoderma harzianum SKD-ZX-1

doi:10.13560/j.cnki.biotech.bull.1985.2019-0586

Abstract

Abstract: Trichoderma harzianum is an important biocontrol bacterium and has an antagonistic effect on pathogenic bacteria of vegetable diseases. In order to determine the biocontrol effect of the isolated T. harzianum SKD-ZX-1,the fermentation broth of the strain was determined and analyzed by GC-MS,and the effect of this strain on the bacterial flora in tomato root rot soil was analyzed by metagenomic sequencing. Further the antagonistic experiment of the strain against Alternaria spp. and Alternaria solani were carried out by plate-to-sputum culture method. The results showed that the fermentation liquid of T. harzianum SKD-ZX-1 contained multiple antibacterial ingredients,namely diisobutyl phthalate,dibutyl phthalate,and di（2-ethylhexyl）phthalate,and increased the types of bacterial flora in the soil. Moreover,Enterobacter increased by 46% in the experimental group compared with the control group,while the genus Pseudomonas and Clostridium decreased by 5.28% and 36.1%,respectively. The inhibition rates of T. harzianum against the two pathogens was 100%,and the inhibition coefficient reached Ⅰ. The present study demonstrates that T. harzianum SKD-ZX-1 has significant inhibitory effect on Alternaria alternata and Alternaria solani,and its fermentation broth has certain antibacterial effect.

Key words: Trichoderma harzianum, antagonism, macrogenomics, GC-MS

LU Hong-sheng ZHANG Xue GAO Yu-ting SUN Pei-ming QIU Meng-meng. Identification,Fermentation and Biocontrol Efficiency of Trichoderma harzianum SKD-ZX-1[J]. Biotechnology Bulletin, 2019, 35(11): 132-140.

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