Abstract: This paper aims to quickly and efficiently identify the authenticity of yak and yak-cattle meat products by PCR-membrane chip technology. We collected samples（8 fresh meat and 19 dry meat samples of fresh pig,goat,oxen,buffalo,yak,chicken,duck,rabbit,dog,and yak-cattle）,extracted and purified DNA,used 11 specific primers to perform multiplex PCR. Further we hybridized the amplified products with a membrane chip containing 12 probes via reverse dot blot,measured the accuracy and sensitivity of the membrane chip,and identified the authenticity of the commercially available yak meat products. The results demonstrated that the membrane chip was of high accuracy,strong specificity,no cross-reaction,and high detection sensitivity（low to 0.1 ng）. Detection results of the commercial fake yak meat products showed that almost all of them were made from yellow cattle and buffalo meat. In conclusion,the application of PCR-membrane chip technology can quickly and accurately identify the real and fake yak meat products,which is conducive to combating counterfeit products and maintaining market balance.

Key words: yak, yak-cattle, multiplex PCR, membrane chip