Molecular Cloning and Tissue Expression of Gene HYOU1

doi:10.13560/j.cnki.biotech.bull.1985.2018-0852

Abstract

Abstract: In order to understand the tissue expression profile of gene HYOU1,the CDS region of HYOU1 in Tibetan yak was cloned and sequenced,and the structure and function of gene HYOU1 were analyzed;furthermore,the mRNA expression profile of gene HYOU1 in the lung,heart,liver,mammary gland,brain and muscle of cattle and yak were detected by RT-PCR. The results were as followings.（1）The CDS region of gene HYOU1 was 3 006 bp,with the percentage of A,G,C and T being 25.0%,31.1%,26.7% and 17.1%,respectively,and there was base preference. One SNP site（ACG→ACA）was identified as a synonymous mutation.（2）Bioinformatics analysis showed that the protein was a neutral,relatively unstable and hydrophilic secretion signal one,and a transmembrane helix（TMhelix）region were obtained and located at the 13-35 amino acid sites. The second and tertiary structures analysis showed that the main spatial structure was random coil and α-helix.（3）Cluster analysis demonstrated that the gene HYOU1 of the Tibetan yak was the closest associated with wild yak,followed by zebu and cattle,and the most distant with buffalo.（4）RT-PCR results indicated that gene HYOU1 expressed in 6 tissues liver,mammary gland,lung,brain,heart,and muscle of cattle and yak at descending order. In addition,for the same tissue,the expression level of the cattle was significantly higher than that of the yak（P<0.05）,i.e.,the expression in the liver of the cattle was 4.4 times higher than that of yak.

Key words: yak, cattle, HYOU1 genes, bioinformatics analysis, tissue expression profile

JI Wen-bo, WANG Hui, CHAI Zhi-xin, WANG Ji-kun, XIN Jin-wei, ZHONG Jin-cheng. Molecular Cloning and Tissue Expression of Gene HYOU1[J]. Biotechnology Bulletin, 2019, 35(3): 123-131.

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