mRNA Expressions of Hypoxia Inducible Factor Genes in Female Yak Reproductive System

doi:10.13560/j.cnki.biotech.bull.1985.2018-0888

Abstract

Abstract: Hypoxia-inducible factor 1α（HIF-1α）and 2α（HIF-2α）are core regulators for inducing hypoxia genes and repairing cellular oxygen environment. This study aims to investigate the adaptation mechanism of the reproduction of yak living on the Qinghai-Tibet Plateau to the hypoxic environment. Hypothalamus,pituitary,oviduct,ovary and uterus tissue samples were collected from five adult and health female yaks and cattle during the follicular phase,respectively. Tissue expression characteristics of these genes were performed by RT-qPCR. The results showed that the mRNA expression level of HIF-1α in the oviduct was the highest,and was very significantly higher in the pituitary and oviduct in yak than in cattle（P<0.01）. Meanwhile,the mRNA expression of HIF-2α was very significantly higher in the hypothalamus（P<0.01）and significantly higher in the ovary of yak than in cattle（P<0.05）. These results suggest that female yaks maintain their reproductive function mainly by regulating HIF-1α and HIF-2α genes in the reproductive system under hypoxic conditions.

Key words: yak, cattle, HIF-1α, HIF-2α, tissue expression

HE Xiang-dong, XIA Yi, JIGE Mo-ti, WANG Hui, ZI Xiang-dong. mRNA Expressions of Hypoxia Inducible Factor Genes in Female Yak Reproductive System[J]. Biotechnology Bulletin, 2019, 35(4): 82-87.

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