Characterization of Rotation Speed on the Fermentation Yield of Clavulanic Acid by Transcriptome Analysis

doi:10.13560/j.cnki.biotech.bull.1985.2019-0954

Abstract

Abstract: The rotation speed affects the fermentation yield of clavulanic acid（CA）. This work aims to explore the molecular mechanism of rotation speed affecting the biosynthesis of CA in Streptomyces clavuligerus F613-1. Transcriptome high-throughput sequencing was adapted to assess the differential gene expressions of CA fermentation conditions between at high rotation speed and low rotation speed,and to analyze the changes in the transcription levels of genes related to the metabolic pathway of CA. The shaking fermentation assay showed that the CA yield at high rotation speed was significantly higher than that at low rotation speed,and the fermentation period of F613-1was shortened. Transcriptome analysis demonstrated that the gene expression level of genes in CA biosynthetic gene cluster had no significant change under the condition of high rotation speed when compared with the low rotation speed. But transcriptome analysis revealed that the expression level of genes involved in metabolism of arginine and glyceraldehyde 3-phosphate（G3P）,two important CA precursors,significantly increased. Therefore,the main reason for the increase of CA biosynthesis in F613-1 under the high rotation speed fermentation condition is the accumulation of CA biosynthetic precursors,arginine and G3P,and the enhancement of arginine and G3P metabolic pathways.

Key words: Streptomyces clavuligerus, clavulanic acid, rotation speed, transcriptome

SONG Dao-ping, KANG Ni, ZHANG Pei-pei, ZONG Gong-li, WANG Shi-li, ZHANG Kun-yu, CAO Guang-xiang, FU Jia-fang. Characterization of Rotation Speed on the Fermentation Yield of Clavulanic Acid by Transcriptome Analysis[J]. Biotechnology Bulletin, 2020, 36(6): 150-156.

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