Study on Antioxidant and Liver Protection of Polysaccharide from Lyophyllum decastes

doi:10.13560/j.cnki.biotech.bull.1985.2021-0891

Abstract

Abstract:

Lyophyllum decastes is a precious edible and medicinal fungus. The crude polysaccharide of Lyophyllum decastes fruit body has many physiological activities such as anti-tumor and liver protection. The free radical scavenging rate was used to analyze the chemical antioxidant activity of L. decastes polysaccharides,and then H₂O₂ was used to establish the LO2 liver cell injury model,and to further study the improvement mechanism of L. decastes fruit body polysaccharides on liver cell oxidative stress damage. The results showed that the polysaccharides from L. decastes fruit body had certain scavenging ability to DPPH,ABTS and OH free radicals in terms of anti-oxidation in vitro,and their EC₅₀ values were:0.218,0.027 and 2.744 mg/mL,respectively. In terms of improving liver cell oxidative stress damage,the cell survival rate also increased significantly as the concentration of polysaccharides increased. The cell survival rate reached 94.1% when the concentration of polysaccharides was 5 mmol/mL. In terms of the mechanism of eliminating the body’s peroxidation,the activities of antioxidant enzymes SOD and CAT increased by 2.55 times and 2.17 times in LDFP group,respectively,compared with the model group,and significantly reduced the level of ROS in cells. The polysaccharide from the fruit body of L. decastes has a certain improvement effect on the oxidative damage of liver cells and provides certain research ideas for the preparation of liver-protecting products of L. decastes.

Key words: Lyophyllum decastes, polysaccharide, antioxidant

ZHANG Feng-pei, XU Hui, QIU Shao-feng, ZHANG Jun-li, WU Xiao-ping, FU Jun-sheng. Study on Antioxidant and Liver Protection of Polysaccharide from Lyophyllum decastes[J]. Biotechnology Bulletin, 2021, 37(11): 92-100.

Figures/Tables 8

Fig.1 Infrared spectrum analysis chart of LDFP

Fig. 2 Ability of LDFP to scavenge free radicals A:OH radical scavenging ability. B:DPPH free radical scavenging ability. C:ABTS radical scavenging ability. Vc is positive control

Fig. 3 Effect of LDFP in different concentration on the cell growth A:Effects of different concentration of polysaccharides on LO2 cell growth. B:Effects of different concentration of polysaccharides on Hepg-2 cell growth. Compared with the blank group,#:P<0.05;##:P<0.01

Fig. 4 Establishment of LO2 cell oxidative damage model A:The effect of different concentrations of H2O2 on the relative survival rate of LO2 cells. Compared with the blank group,#:P<0.05;##:P<0.01. B:The effect of different concentrations of H2O2 on the morphology of LO2 cells （a:control group;b:0.05 mmol/L;c:1 mmol/L;d:8 mmol/L）

Fig. 5 Protective effect of LDFP at different concentrations on oxidative damage Compared with the blank group,#:P<0.05;##:P<0.01. Compared with model group,*:P<0.05;**:P<0.01;the same below

Fig. 6 Ability of LDFP to scavenge ROS produced by oxidative damage of liver cells

Fig. 7 Regulation of LDFP on the expression of SOD enzyme activity in cells

Fig. 8 Regulation of LDFP on the expression of CAT enzyme activity in cells

References 34

[1]	张凌姗. 鹿茸菇生态学特性与液体菌种配方的优化研究[J]. 食药用菌, 2020, 28(6): 425-427, 439.
	Zhang LS. Study on ecological characteristics and optimization of liquid spawn formula of Lyophyllum decastes[J]. Edible Med Mushrooms, 2020, 28(6): 425-427, 439.
[2]	程继红. 名贵食药用菌鹿茸菇商业化生产现状与发展前景[J]. 食药用菌, 2014, 22(4): 194-197.
	Cheng JH. The commercial production situation and prospects of the new valuable edible and medicinal mushroom, Lyophyllum decastes[J]. Edible Med Mushrooms, 2014, 22(4): 194-197.
[3]	秦春青. 鹿茸菇子实体多糖提取分离、结构鉴定及抗氧化研究[D]. 杭州:浙江工业大学, 2019.
	Qin CQ. Study on Extraction, Isolation, Structure Identification and Antioxidant of Polysaccharides from the Fruit Body of Lyophyllum decastes[D]. Hangzhou:Zhejiang University of Technology, 2019.
[4]	Ukawa Y, Ito H, Hisamatsu M. Antitumor effects of(1→3)-β-d-glucan and(1→6)-β-d-glucan purified from newly cultivated mushroom, Hatakeshimeji(Lyophyllum decastes Sing. )[J]. J Biosci Bioeng, 2000, 90(1): 98-104. pmid: 16232825
[5]	闫肃. 荷叶离褶伞子实体化学成分与生物活性研究[D]. 长春:吉林农业大学, 2014.
	Yan S. Studies on chemical components and biological activity of Lyophyllum decastes(Fr. :Fr. )Sing[D]. Changchun:Jilin Argicultural University, 2014
[6]	商佳琦, 邹丹阳, 滕翔宇, 等. 5种食用菌多糖的结构特征及抗氧化活性对比[J]. 食品工业科技, 2020, 41(15): 77-83, 89.
	Shang JQ, Zou DY, Teng XY, et al. Structural characterization and antioxidant activity of five kinds of edible fungus polysaccharides[J]. Sci Technol Food Ind, 2020, 41(15): 77-83, 89.
[7]	王帅, 赵冬雪, 韩成凤, 等. 6种活性多糖的结构、性质及其抗氧化活性的比较研究[J]. 食品研究与开发, 2021, 42(16): 7-15.
	Wang S, Zhao DX, Han CF, et al. A comparative study on the structure, properties and antioxidant activity of six active polysaccharides[J]. Food Res Dev, 2021, 42(16): 7-15.
[8]	Huang XF, Liu ZG, Lei P, et al. Protective effect of saikosaponin-d on H₂O₂-induced hepatocellular injury and its mechanism[J]. 中国药理学与毒理学杂志, 2021, 35(3): 169-175.
	Huang XF, Liu ZG, Lei P, et al. Protective effect of saikosaponin-d on H₂O₂-induced hepatocellular injury and its mechanism[J]. Chin J Pharmacol Toxicol, 2021, 35(3): 169-175.
[9]	刘城移, 袁源, 等. 蝉虫草菌丝体胞内和胞外多糖抗肝细胞氧化损伤比较研究[J]. 菌物学报, 2020, 39(2): 421-433.
	Liu CY, Yuan Y, et al. A comparative study of intracellular and extracellular polysaccharides in mycelia of Cordyceps cicadae against oxidative damage of hepatocytes[J]. Mycosystema, 2020, 39(2): 421-433.
[10]	刘鑫, 李琳, 陈磊, 等. 利用扁桃斑鸠菊叶发酵高产粗毛纤孔菌胞外多糖的条件优化及其抗氧化活性研究[J]. 菌物学报, 2019, 38(3): 403-413.
	Liu X, Li L, Chen L, et al. Optimization on fermentation conditions for high production of Inonotus hispidus exopolysaccharides using Vernonia amygdalina dried leaf powder as additive and analyses on antioxidant activities of the polysaccharide product[J]. Mycosystema, 2019, 38(3): 403-413.
[11]	郝金斌, 孟国良, 吴龙月, 等. 奶油栓孔菌的鉴定及其生物学特性[J]. 西北农林科技大学学报:自然科学版, 2021, 49(8): 133-144.
	Hao JB, Meng GL, Wu LY, et al. Identification and biological characteristics of Trametes lactinea[J]. J Northwest A F Univ:Nat Sci Ed, 2021, 49(8): 133-144.
[12]	Cui Q, Sun YX, Cheng JJ, et al. Effect of two-step enzymatic hydrolysis on the antioxidant properties and proteomics of hydrolysates of milk protein concentrate[J]. Food Chem, 2022, 366: 130711. doi: 10.1016/j.foodchem.2021.130711 URL
[13]	张桂芳, 张东杰, 鹿保鑫, 等. 不同萌发时间黑豆芽总黄酮含量及DPPH自由基清除能力的测定[J]. 食品科技, 2020, 45(12): 39-45.
	Zhang GF, Zhang DJ, Lu BX, et al. Determination of total flavonoid content and DPPH free radical scavenging ability of black bean sprouts at different germination times[J]. Food Sci Technol, 2020, 45(12): 39-45.
[14]	Shang HM, Zhou HZ, Yang JY, et al. In vitro and in vivo antioxidant activities of inulin[J]. PLoS One, 2018, 13(2): e0192273. doi: 10.1371/journal.pone.0192273 URL
[15]	金爱花, 常慧, 张钊, 等. 草苁蓉多糖对过氧化氢致HL-7702细胞损伤的保护作用[J]. 食品与发酵工业, 2021. DOI: DOI 10. 13995/j. cnki. 11-1802/ts. 026921. doi: 10
	Jin AH, Chang H, Zhang Z, et al. Protective effect of Boschniakia rossica polysaccharides on H₂O₂-induced injury of HL-7702 cells[J]. Food and Fermentation Industries, 2021. DOI: 10.13995/j.cnki.11-1802/ts.026921. doi: 10.13995/j.cnki.11-1802/ts.026921
[16]	马广明, 刘骥, 姜鑫, 等. 辣木叶多糖对过氧化氢诱导奶牛乳腺上皮细胞氧化损伤的保护作用[J]. 动物营养学报, 2021, 33(1): 350-358.
	Ma GM, Liu J, Jiang X, et al. Protective effects of Moringa leaf polysaccharide on oxidative damage of dairy cow mammary epithelial cells induced by hydrogen peroxide[J]. Chin J Animal Nutr, 2021, 33(1): 350-358.
[17]	龚雯, 唐婕, 韦雅渊, 等. 金花茶多糖分离纯化、结构表征及其体外抗氧化性[J]. 食品与机械, 2021, 37(6): 184-190.
	Gong W, Tang J, Wei YY, et al. Isolation, purification, structural characterization of polysaccharide from Camellia nitidissima Chi and its antioxidant activities in vitro[J]. Food & Machinery, 2021, 33(1): 350-358.
[18]	陈建平, 陈曦, 钟赛意, 等. 番茄红素对人体血清自由基的清除作用[J]. 食品工业科技, 2018, 39(24): 309-312, 317.
	Chen JP, Chen X, Zhong SY, et al. Scavenging effect of lycopene on free radical of human serum[J]. Sci Technol Food Ind, 2018, 39(24): 309-312, 317.
[19]	谭曜, 方爱琴, 谢建敏, 等. 蜂胶乙醇溶液体外清除羟基自由基能力的测定[J]. 广东化工, 2021, 48(12): 189-190, 182.
	Tan Y, Fang AQ, Xie JM, et al. Study on effect of Propolis ethanol solution on scavenging hydroxyl radical in vitro[J]. Guangdong Chem Ind, 2021, 48(12): 189-190, 182.
[20]	Treml J, Šmejkal K. Flavonoids as potent scavengers of hydroxyl radicals[J]. Compr Rev Food Sci Food Saf, 2016, 15(4): 720-738. doi: 10.1111/crf3.2016.15.issue-4 URL
[21]	朱杰, 张华南, 范毅, 等. 响应面优化亚麻荠籽多糖提取工艺及其体外抗氧化活性研究[J]. 中国油脂, 2021, 46(10): 127-131.
	Zhu J, Zhang HN, Fan Y, et al. Optimization of extraction process of polysaccharides from Camelina sativa(L.)Crantz seeds meal with response surface methodology and its antioxidant activity[J]. China Oils and Fats, 2021, 46(10): 127-131.
[22]	Ionita P. The chemistry of DPPH· free radical and congeners[J]. Int J Mol Sci, 2021, 22(4): 1545. doi: 10.3390/ijms22041545 URL
[23]	李生茂, 李倩茹, 张馨予, 等. 响应面法优化益智总黄酮超声辅助提取工艺及其抗氧化活性评价[J]. 保鲜与加工, 2021, 31(8): 43-49.
	Li SM, Li QR, Zhang XY, et al. Optimization of μLtrasound-Assisted technology and antioxidant activity of total flavonoids of Alpinia oxyphylla by response surface methodology[J]. Storage and Process \| Stor Proc, 2021, 31(8): 43-49.
[24]	Song W, Yuan Y, Yu N, et al. Antioxidant capacity of extract from Jiangtang Xiaozhi recipe in vitro[J]. J Tradit Chin Med, 2020, 40(3): 393-400.
[25]	马传贵, 张志秀, 鲍文辉. 食用菌多糖及其生物活性的研究进展[J]. 食药用菌, 2021, 29(3): 196-201.
	Ma CG, Zhang ZX, Bao WH. Research progress on extraction and bioactivity of polysaccharides from edible mushrooms[J]. Edible Med Mushrooms, 2021, 29(3): 196-201.
[26]	胡文森, 刘义, 任勰珂, 等. 食用菌中功能性物质的研究进展[J]. 现代食品, 2020(24): 34-37.
	Hu WS, Liu Y, Ren XK, et al. Research progress of functional substances in edible fungi[J]. Mod Food, 2020(24): 34-37.
[27]	江和栋, 牛仙, 万仁口, 等. 灵芝孢子多糖的提取工艺优化及单糖组成、抗氧化活性分析[J]. 中国食品学报, 2021, 21(4): 159-167.
	Jiang HD, Niu X, Wan RK, et al. Optimization of extraction and analysis of monosaccharide composition and antioxidant activity of Ganoderma lucidum spore polysaccharide[J]. J Chin Inst Food Sci Technol, 2021, 21(4): 159-167.
[28]	郭璐, 王雅葳, 张少冰, 等. 忍冬桑黄和蛹虫草共发酵联产真菌多糖初步研究[J]. 菌物学报, 2021, 40(4): 965-976.
	Guo L, Wang YW, Zhang SB, et al. A Sanghuangporus lonicericola and Cordyceps militaris to produce fungal polysaccharides[J]. Mycosystema, 2021, 40(4): 965-976.
[29]	程继红. 鹿茸菇的栽培现状与营养保健价值[J]. 食药用菌, 2021, 29(1): 12-15.
	Cheng JH. Cultivation status and its nutrition and health care value of Lyophyllum decastes[J]. Edible Med Mushrooms, 2021, 29(1): 12-15.
[30]	戚梦, 刘城移, 等. 大革耳子实体多糖抗氧化活性[J]. 菌物学报, 2018, 37(12): 1707-1716.
	Qi M, Liu CY, et al. Polysaccharide antioxidant activities of Panus giganteus[J]. Mycosystema, 2018, 37(12): 1707-1716.
[31]	Abdel-Rahman EA, Hosseiny S, Aaliya A, et al. Sleep/wake calcium dynamics, respiratory function, and ROS production in cardiac mitochondria[J]. J Adv Res, 2021, 31: 35-47. doi: 10.1016/j.jare.2021.01.006 pmid: 34194831
[32]	Zorov DB, Juhaszova M, Sollott SJ. Mitochondrial reactive oxygen species(ROS)and ROS-induced ROS release[J]. Physiol Rev, 2014, 94(3): 909-950. doi: 10.1152/physrev.00026.2013 URL
[33]	严苏晴, 俞晓卫, 郭静科, 等. SOD强化奶片的抗氧化性及对人体经络电压的作用[J]. 中国食品学报, 2021, 21(6): 107-113.
	Yan SQ, Yu XW, Guo JK, et al. The anti-oxidative capacity of milk tablet enriched with SOD(MKTES)and its effect on human electrical potential[J]. J Chin Inst Food Sci Technol, 2021, 21(6): 107-113.
[34]	Inoue M, Watanabe N, Utsumi T, et al. Targeting SOD by gene and protein engineering and inhibition of free radical injury[J]. Free Radic Res Commun, 1991, :391-399.