Effects of Dendrobium officinale Polysaccharides on the Intestinal Mucosal Structure and Microbiota in Mice Fed a High-fat Diet

doi:10.13560/j.cnki.biotech.bull.1985.2021-0390

Abstract

Abstract:

To investigate the effects of Dendrobium officinale polysaccharides（DOP）on the intestinal mucosal barrier of mice fed a high fat diet,DOP were extracted by water extraction and alcohol precipitation method. DOP were gavaged with the mice fed a high fat diet for 8 weeks,and the intestinal mucosal structure and intestinal mucosal microbiota of mice were observed. The results showed that high fat diet significantly destroyed the intestinal mucosal structure,which was characterized by intestinal mucosal atrophy,epithelial cells shedding and inflammatory exudation. Moreover,infection-associated and inflammation-related bacteria,for example,Corynebacterium_1 and Staphylococcus proliferated greatly. DOP protected intestinal mucosal structure. In addition,DOP reduced the abundance of Corynebacterium_1,increased the abundance of Candidatus_Arthromitus,and enhanced the proliferation of bacteria related to carbohydrate metabolism and short-chain fatty acid production,i.e.,Muribaculaceae,Bacteroides and Lachnospiraceae_NK4A136_group. The results showed that DOP could protect the intestinal mucosal barrier by maintaining the structural integrity of intestinal mucosa,regulating the composition of intestinal mucosal microbiota,promoting carbohydrate metabolism,and producing short-chain fatty acids.

Key words: Dendrobium officinale polysaccharides, high fat diet, intestinal mucosa, microbiota, diversity

XIE Guo-zhen, TANG Yuan, NING Xiao-mei, QIU Ji-hui, TAN Zhou-jin. Effects of Dendrobium officinale Polysaccharides on the Intestinal Mucosal Structure and Microbiota in Mice Fed a High-fat Diet[J]. Biotechnology Bulletin, 2022, 38(2): 150-157.

Figures/Tables 6

Fig. 1 Effects of DOP on the intestinal mucosal structure of mice fed a high fat diet N：Normal group. H：High fat diet group. HP：DOP group. The same below

Fig. 2 Effect of DOP on the OTU of mucosa-associated microbiota of mice fed a high fat diet

Fig. 3 Effect of DOP on the Alpha diversity of mucosa-associated microbiota of mice fed a high fat diet（±s,N = 5） * refers to HP compared with N,P<0.05

Fig. 4 Effect of DOP on the Beta diversity of mucosa-assoc-iated microbiota of mice fed a high fat diet（N = 5）

Fig. 5 Effect of DOP on the structure of mucosa-associated microbiota of mice fed a high fat diet A：Phylum. B：Genus

Fig. 6 Biomarker of mucosa-associated microbiota after DOP and high-fat diet treating

References 33

[1]	Wan MLY, Ling KH, El-Nezami H, et al. Influence of functional food components on gut health[J]. Crit Rev Food Sci Nutr, 2019, 59(12):1927-1936. doi: 10.1080/10408398.2018.1433629 URL
[2]	Gulhane M, Murray L, Lourie R, et al. High fat diets induce colonic epithelial cell stress and inflammation that is reversed by IL-22[J]. Sci Rep, 2016, 6:28990. doi: 10.1038/srep28990 pmid: 27350069
[3]	Proctor C, Thiennimitr P, Chattipakorn N, et al. Diet, gut microbiota and cognition[J]. Metab Brain Dis, 2017, 32(1):1-17. doi: 10.1007/s11011-016-9917-8 pmid: 27709426
[4]	Zhang M, Yang XJ. Effects of a high fat diet on intestinal microbiota and gastrointestinal diseases[J]. World J Gastroenterol, 2016, 22(40):8905-8909. doi: 10.3748/wjg.v22.i40.8905 URL
[5]	Nguyen SG, Kim J, Guevarra RB, et al. Laminarin favorably modulates gut microbiota in mice fed a high-fat diet[J]. Food Funct, 2016, 7(10):4193-4201. pmid: 27713958
[6]	Shang QS, Wang Y, Pan L, et al. Dietary polysaccharide from Enteromorpha clathrata modulates gut microbiota and promotes the growth of Akkermansia muciniphila, Bifidobacterium spp. and Lactobacillus spp[J]. Mar Drugs, 2018, 16(5):167. doi: 10.3390/md16050167 URL
[7]	Shi HJ, Chang YG, Gao Y, et al. Dietary fucoidan of Acaudina molpadioides alters gut microbiota and mitigates intestinal mucosal injury induced by cyclophosphamide[J]. Food Funct, 2017, 8(9):3383-3393. doi: 10.1039/C7FO00932A URL
[8]	Kanwal S, Joseph TP, Owusu L, et al. A polysaccharide isolated from Dictyophora indusiata promotes recovery from antibiotic-driven intestinal dysbiosis and improves gut epithelial barrier function in a mouse model[J]. Nutrients, 2018, 10(8):1003. doi: 10.3390/nu10081003 URL
[9]	蔡光先, 李娟, 李顺祥, 等. 铁皮石斛古代与现代的应用概况[J]. 湖南中医药大学学报, 2011, 31(5):77-81.
	Cai GX, Li J, Li SX, et al. Applications of Dendrobium officinale in ancient and modern times[J]. J Tradit Chin Med Univ Hunan, 2011, 31(5):77-81.
[10]	Zeng Q, Ko CH, Siu WS, et al. Polysaccharides of Dendrobium officinale Kimura & Migo protect gastric mucosal cell against oxidative damage-induced apoptosis in vitro and in vivo[J]. J Ethnopharmacol, 2017, 208:214-224. doi: 10.1016/j.jep.2017.07.006 URL
[11]	Liang J, Chen S, Chen J, et al. Therapeutic roles of polysaccharides from Dendrobium Officinale on colitis and its underlying mechanisms[J]. Carbohydr Polym, 2018, 185:159-168. doi: 10.1016/j.carbpol.2018.01.013 URL
[12]	中华人民共和国药典委员会. 中华人民共和国药典一部[S]. 北京: 中国医药科技出版社, 2020:295-296.
	Chinese Pharmacopoeia Commission. Pharmacopoeia of the People’s Republic of China(No. 1). Beijing: China Medical Science and Technology Press, 2020:295-296.
[13]	Vancamelbeke M, Vermeire S. The intestinal barrier:a fundamental role in health and disease[J]. Expert Rev Gastroenterol Hepatol, 2017, 11(9):821-834. doi: 10.1080/17474124.2017.1343143 URL
[14]	Camilleri M, Madsen K, Spiller R, et al. Intestinal barrier function in health and gastrointestinal disease[J]. Neurogastroenterol Motil, 2012, 24(6):503-512. doi: 10.1111/nmo.2012.24.issue-6 URL
[15]	Schroeder BO, Birchenough GMH, Ståhlman M, et al. Bifidobacteria or fiber protects against diet-induced microbiota-mediated colonic mucus deterioration[J]. Cell Host Microbe, 2018, 23(1):27-40. e7. doi: S1931-3128(17)30498-5 pmid: 29276171
[16]	Ding SL, Lund PK. Role of intestinal inflammation as an early event in obesity and insulin resistance[J]. Curr Opin Clin Nutr Metab Care, 2011, 14(4):328-333. doi: 10.1097/MCO.0b013e3283478727 URL
[17]	Borgo F, Garbossa S, Riva A, et al. Body mass index and sex affect diverse microbial niches within the gut[J]. Front Microbiol, 2018, 9:213. doi: 10.3389/fmicb.2018.00213 URL
[18]	Chen WG, Liu FL, Ling ZX, et al. Human intestinal lumen and mucosa-associated microbiota in patients with colorectal cancer[J]. PLoS One, 2012, 7(6):e39743. doi: 10.1371/journal.pone.0039743 URL
[19]	Liguori G, Lamas B, Richard ML, et al. Fungal dysbiosis in mucosa-associated microbiota of Crohn’s disease patients[J]. J Crohns Colitis, 2016, 10(3):296-305. doi: 10.1093/ecco-jcc/jjv209 URL
[20]	Galley JD, Nelson MC, Yu Z, et al. Exposure to a social stressor disrupts the community structure of the colonic mucosa-associated microbiota[J]. BMC Microbiol, 2014, 14:189. doi: 10.1186/1471-2180-14-189 URL
[21]	Dalen G, Rachah A, Nørstebø H, et al. Transmission dynamics of intramammary infections caused by Corynebacterium species[J]. J Dairy Sci, 2018, 101(1):472-479. doi: 10.3168/jds.2017-13162 URL
[22]	Costales J, Alsyouf M, Napolitan P, et al. Corynebacterium urealyt-icum:rare urinary tract infection with serious complications[J]. Can J Urol, 2019, 26(1):9680-9682. pmid: 30797252
[23]	Abbott Y, Efstratiou A, Brennan G, et al. Toxigenic Corynebacterium ulcerans associated with upper respiratory infections in cats and dogs[J]. J Small Anim Pract, 2020, 61(9):554-560. doi: 10.1111/jsap.13185 pmid: 32734615
[24]	Das S, Rao AS, Sahu SK, et al. Corynebacterium spp as causative agents of microbial keratitis[J]. Br J Ophthalmol, 2016, 100(7):939-943. doi: 10.1136/bjophthalmol-2015-306749 URL
[25]	Ogasawara M, Matsuhisa T, Kondo T, et al. Pyogenic spondylitis with acute course caused by Corynebacterium simulans[J]. J Infect Chemother, 2020, 26(3):294-297. doi: S1341-321X(19)30329-0 pmid: 31735633
[26]	Ondusko DS, Nolt D. Staphylococcus aureus[J]. Pediatr Rev, 2018, 39(6):287-298. doi: 10.1542/pir.2017-0224 URL
[27]	Hedblom GA, Reiland HA, Sylte MJ, et al. Segmented filamentous bacteria - metabolism meets immunity[J]. Front Microbiol, 2018, 9:1991. doi: 10.3389/fmicb.2018.01991 pmid: 30197636
[28]	Chung YW, Gwak HJ, Moon S, et al. Functional dynamics of bacterial species in the mouse gut microbiome revealed by metagenomic and metatranscriptomic analyses[J]. PLoS One, 2020, 15(1):e0227886. doi: 10.1371/journal.pone.0227886 URL
[29]	Tan HZ, Zhai QX, Chen W. Investigations of Bacteroides spp. towards next-generation probiotics[J]. Food Res Int, 2019, 116:637-644. doi: 10.1016/j.foodres.2018.08.088 URL
[30]	Li ZR, Jia RB, Wu J, et al. Sargassum fusiforme polysaccharide partly replaces acarbose against type 2 diabetes in rats[J]. Int J Biol Macromol, 2021, 170:447-458. doi: 10.1016/j.ijbiomac.2020.12.126 URL
[31]	Dong J, Liang Q, Niu Y, et al. Effects of Nigella sativa seed polysaccharides on type 2 diabetic mice and gut microbiota[J]. Int J Biol Macromol, 2020, 159:725-738. doi: 10.1016/j.ijbiomac.2020.05.042 URL
[32]	Wang L, Li C, Huang Q, et al. Polysaccharide from Rosa roxburghii tratt fruit attenuates hyperglycemia and hyperlipidemia and regulates colon microbiota in diabetic db/db mice[J]. J Agric Food Chem, 2020, 68(1):147-159. doi: 10.1021/acs.jafc.9b06247 URL
[33]	Stadlbauer V, Engertsberger L, Komarova I, et al. Dysbiosis, gut barrier dysfunction and inflammation in dementia:a pilot study[J]. BMC Geriatr, 2020, 20(1):248. doi: 10.1186/s12877-020-01644-2 pmid: 32690030