Antimony Accumulation and Its Effect on Antioxidation System in Different Tissues of Danio rerio

doi:10.13560/j.cnki.biotech.bull.1985.2020-1060

Abstract

Abstract:

This study aimed to study the accumulation of antimony in zebrafish tissues and changes in various enzyme activities and contents under the stress of different concentration of antimony. Danio rerio were exposed to antimony solution（0,13.26,26.53,and 39.79 mg/L）for 14 d to determine the accumulation of antimony,the activity of superoxide dismutase（SOD）,catalase（CAT）,content of glutathione（GSH）and malondialdehyde（MDA）in each tissue. The results showed that the order of antimony accumulation in each tissue was as：Liver > Gill> Muscle > Brain and it gradually increased with the increase of antimony stress concentration. With the increased of antimony stress concentration,SOD activity in the liver,brain,gills and muscles presented different trends;CAT activity and GSH content both increased first and then decreased,which was as promotion at low concentration and inhibition at high concentration;MDA content tended gradually increasing. In conclusion,antimony can accumulate in different tissues of Danio rerio,activating antioxidant defense system and causing lipid peroxidation.

Key words: antimony, zebrafish, accumulation, antioxidant, lipid peroxidation

XU Kun, YANG Ai-jiang, HU Xia, ZOU Hai-tao, LI Bin, LIU Ji. Antimony Accumulation and Its Effect on Antioxidation System in Different Tissues of Danio rerio[J]. Biotechnology Bulletin, 2021, 37(4): 145-154.

Figures/Tables 5

Fig.1 Total antimony content in liver, brain, gill and muscle of zebrafish under different concentrations of antimony treatment The concentration in the control group was ND (not detected), and different letters indicate significant differences between the groups (P<0.05),the same below(n=3)

Fig.2 SOD activity in liver, brain, gill and muscle of zebrafish under different concentrations of antimony treatment

Fig.3 SOD activity in liver, brain, gill and muscle of zebrafish under different concentrations of antimony treatment

Fig.4 GSH content in liver, brain, gill and muscle of zebrafish under different concentrations of antimony treatment

Fig.5 MDA content in liver, brain, gill and muscle of zebrafish under different concentrations of antimony treatment

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