实时荧光定量PCR技术在肠道微生物领域中的研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2014.12.010

摘要/Abstract

摘要： 实时荧光定量 PCR 技术是目前发展起来的快速、精确定量核酸的最有效的方法之一，是生物定量分析的强有力的手段。与宏基因组测序等测序技术相比，实时荧光定量 PCR 技术能确定出样品中菌体的具体数量，同时具有操作简便、快速高效、特异性强、高通量等特点，因此被广泛应用于肠道微生物领域中。近年来，在肠道微生物和疾病的相关研究中，采用实时荧光定量 PCR 技术已成为趋势。综述了近几年实时荧光定量 PCR 在肠道微生物多样性和饮食干预在微生物菌群的基因组成方面的研究进展。

关键词: 实时荧光定量, PCR, 肠道菌群, 微生物多样性

Abstract: Real-time PCR is one of the most effective methods, which rapidly accurate quantify nucleic acids. Compared with metagenomic, real-time quantitative PCR can quantify microorganism . Real-time PCR, a simple, fast, efficient, specific and high-through put method, is widely used in the field of gut microbiology. The technology in studies of intestinal microbes and disease has a wide range of applications. This paper reviews research progress about real-time PCR in the field of intestinal microbial recent years.

Key words: Real-time PCR, Gut microbiota, Microbiota diversity

赵洁 ,马晨 ,席晓敏, 张和平. 实时荧光定量PCR技术在肠道微生物领域中的研究进展[J]. 生物技术通报, 2014, 0(12): 61-66.

Zhao Jie, Ma Chen, Xi Xiaomin, Zhang Heping. Advances of Real-time PCR Technology in the Field of Gut Microbiota[J]. Biotechnology Bulletin, 2014, 0(12): 61-66.

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