产毒真菌基因组研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2015.02.004

生物技术通报 ›› 2015, Vol. 31 ›› Issue (2): 26-34.doi: 10.13560/j.cnki.biotech.bull.1985.2015.02.004

产毒真菌基因组研究进展

王龑^1，2, 刘阳^1，2, 刘伊宁¹

1. 中国农业科学院农产品加工研究所，北京 100193;
2. 农业部农产品加工重点实验室，北京 100193

收稿日期:2014-11-02 出版日期:2015-02-05 发布日期:2015-02-06
作者简介:王龑，女，博士，助理研究员，研究方向：农产品真菌毒素预防控制；E-mail：wangyan062006@163.com
基金资助:
国家“973”计划项目（2013CB127805），国家公益性行业（农业）科研专项（201203037），科技基础性工作专项（2013FY113400）

Advances in Studies on Genomics of Toxogenic Fungi

Wang Yan^1，2, Liu Yang^1，2, Liu Yining¹

1. Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193； 2. Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing 100193

Received:2014-11-02 Published:2015-02-05 Online:2015-02-06

摘要/Abstract

摘要： 真菌毒素是产毒真菌产生的次生代谢产物，以木霉、曲霉、毛霉、青霉和根霉为主的丝状真菌是农产品中常见的产毒真菌。阐明农产品中产毒致病微生物的基因组序列信息是揭示真菌特殊遗传性状的基础。截至2013年12月，共有子囊菌门中204个种和担子菌门中62个种的基因组序列已经测序或公布，它们的基因组大小大部分在30-40 Mb。整理了部分已完成基因组测序的具有产毒能力或致病力的真菌基因组信息，并对真菌测序方案、主要产毒真菌及其比较基因组学的研究进展进行了综述。

关键词: 真菌, 真菌毒素, 农产品, 基因组, 比较基因组学

Abstract: Mycotoxins, a group of toxins structurally related to secondary metabolites are produced by some fungal strains. It is mainly produced by filamentous fungi, including Trichoderma, Aspergillus, Chaetomium, Penicillium and Rhizopus, existed in agricultural products. Illustrating the genome sequence information of the pathogenic microorganisms in agricultural products, is essential to reveal fungal specific genetic traits. Until to December 2013, a total of 204 species in Ascomycetes and 62 species in Basidiomycete genomic information had been sequenced or published, genomes size from approximately 30 Mb to 40 Mb. This review here provides an overview of available genomic information of toxin-producing fungal or virulent fungus, summarizes the recent development in genome sequencing strategies and main fungal produced mycotoxin, and proposes the future direction of the comparative genomics research on fungi.

Key words: fungi, mycotoxin, agricultural products, genome, comparative genomics

王龑, 刘阳, 刘伊宁. 产毒真菌基因组研究进展[J]. 生物技术通报, 2015, 31(2): 26-34.

Wang Yan, Liu Yang, Liu Yining. Advances in Studies on Genomics of Toxogenic Fungi[J]. Biotechnology Bulletin, 2015, 31(2): 26-34.

参考文献

[1]Bennett JWKM. Mycotoxins[J]. Clin Microbiol Rev, 2003, 16：497-516.
[2]Hussein HS, Brasel JM. Toxicity, metabolism, and impact of mycotoxins on humans and animals[J]. Toxicology, 2001, 167：101-134.
[3]Chiang YM, Szewczyk E, Davidson AD, et al. A gene cluster containing two fungal polyketide synthases encodes the biosynthetic pathway for apolyketide, asperfuranone, in Aspergillus nidulans[J]. Journal of the American Chemical Society, 2009, 131：2965-2970.
[4]Pel HJ, De Winde JH, Archer DB, et al. Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88[J]. Nature Biotechnology, 2007, 25：221-231.
[5]孙健冬, 高通量测序技术在农作物全基因组序列测定中的应用概览[J]. 生物技术进展, 2012, 2（1）：11-15.
[6]王龑, 许文涛, 赵维薇, 等. 组学技术及其在食品科学中应用的研究进展[J]. 生物技术通报, 2011（11）：005.
[7]刘岩, 吴秉铨. 第三代测序技术：单分子即时测序[J]. 中华病理学杂志, 2011, 40（10）：718-720.
[8]Galagan JE, Calvo SE, Borkovich KA, et al. The genome sequence of the filamentous fungus Neurospora crassa[J]. Nature, 2003, 422：859-868.
[9]Wood V, Gwilliam R, Rajandream MA, et al. The genome sequence of Schizosaccharomyces pombe[J]. Nature, 2002, 415：871-880.
[10]余知和, 高元钢, 曾昭清, 等. 真菌基因组学研究进展[J]. 菌物学报, 2008, 27（5）：778-787.
[11]Nierman WC, Pain A, Anderson MJ, et al. Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus[J]. Nature, 2005, 438：1151-1156.
[12]Machida M, Asai K, Sano M, et al. Genome sequencing and analysis of Aspergillus oryzae[J]. Nature, 2005, 438：1157-1161.
[13]Galagan JE, Calvo SE, Cuomo C, et al. Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae[J]. Nature, 2005, 438：1105-1115.
[14]Van den Berg MA, Albang R, Albermann K, et al. Genome sequencing and analysis of the filamentous fungus Penicillium chrysogenum[J]. Nature Biotechnology, 2008, 26：1161-1168.
[15]Andersen MR, Salazar MP, Schaap PJ, et al. Comparative genomics of citric-acid-producing Aspergillus niger ATCC 1015 versus enzyme-producing CBS 513.88[J]. Genome Research, 2011, 21：885-897.
[16]Ma LJ, Van Der Does HC, Borkovich KA, et al. Comparative genomics reveals mobile pathogenicity chromosomes in Fusarium[J]. Nature, 2010, 464：367-373.
[17]Fedorova ND, Khaldi N, Joardar VS, et al. Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus[J]. Plos Genetics, 2008, 4：e1000046.
[18]Wilkinson B, Micklefield J. Mining and engineering natural-product biosynthetic pathways[J]. Nature Chemical Biology, 2007, 3：379-386.
[19] Gross H. Genomic mining-a concept for the discovery of new bioactive natural products[J]. Current Opinion In Drug Discovery & Development, 2009, 12：207-219.
[20] Bergmann S, Schümann J, Scherlach K, et al. Genomics-driven discovery of PKS-NRPS hybrid metabolites from Aspergillus nidulans[J]. Nature Chemical Biology, 2007, 3：213-217.
[21] Gallo A, Knox BP, Bruno KS, et al. Identification and characteriz-ation of the polyketide synthase involved in ochratoxin A biosynthe-sis in Aspergillus carbonarius[J]. International Journal of Food Microbiology, 2014, 179：10-17.
[22] Yu J. Current understanding on aflatoxin biosynthesis and future perspective in reducing aflatoxin contamination[J]. Toxins, 2012, 4：1024-1057.
[23] Brakhage AA, Schroeckh V. Fungal secondary metabolites strategies to activate silent gene clusters[J]. Fungal Genetics and Biology, 2011, 48：15-22.
[24] O’Callaghan J, Coghlan A, Abbas A, et al. Functional characterization of the polyketide synthase gene required for ochratoxin A biosynthesis in Penicillium verrucosum[J]. International Journal of Food Microbiology, 2013, 161：172-181.
[25] Atoui A, Phong Dao H, Mathieu F, et al. Amplification and diversity analysis of ketosynthase domains of putative polyketide synthase genes in Aspergillus ochraceus and Aspergillus carbonarius producers of ochratoxin A[J]. Molecular Nutrition & Food Research, 2006, 50：488-493.
[26] Bhatnagar D, Rajasekaran K, Payne G, et al. The ‘omics’ tools：genomics, proteomics, metabolomics and their potential for solving the aflatoxin contamination problem[J]. World Mycotoxin Journal, 2008, 1：3-12.
[27] Yu J, Ronning CM, Wilkinson JR, et al. Gene profiling for studying the mechanism of aflatoxin biosynthesis in Aspergillus flavus and A. parasiticus[J]. Food Additives and Contaminants, 2007, 24：1035-1042.
[28] Yu J, Fedorova ND, Montalbano BG, et al. Tight control of mycotoxin biosynthesis gene expression in Aspergillus flavus by temperature as revealed by RNA-Seq[J]. FEMS Microbiology Letters, 2011, 322：145-149.

产毒真菌基因组研究进展

Advances in Studies on Genomics of Toxogenic Fungi

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