关键词: 常压室温等离子体诱变（ARTP）, 哈茨木霉, 农药抗性, 驯化选育

Abstract: This study aimed to improve the adaptability of biocontrol Trichoderma in the complicated agricultural environment in where herbicides, insecticides, and fungicides co-exist. Trichoderma harzianum was used as initial strain and induced by atmospheric and room temperature plasma（ARTP）. The Trichoderma harzianum mutants resistant to both fungicides mancozeb and insecticide dinotefuran were obtained and one with the most double-resistance was named as Th-36. The mutant was subject to further mutation by ARTP and screened on the medium with mancozeb, dinotefuran and herbicide oxyfluorfen. Two mutants with triple-resistance were obtained and named as Th-3-36 and Th-4-B. The toxicity resistance test showed that EC₅₀s of mancozeb for Th-3-36 and Th-4-B were up to 5089.2 μg a.i.·mL^-1 and 5498.2 μg a.i.·mL^-1；for dinotefuran up to 758.5 μg a.i.·mL^-1 and 785.9 μg a.i.·mL^-1；for oxyfluorfen up to 198.2 μg a.i.·mL^-1 and 200.3 μg a.i.·mL^-1 respectively. The above values were all higher than the average commercially recommended doses of these pesticides. The screened mutants showed the significant increasing resistance to pesticide, inherited parental broad-spectrum antibacterial property, and presented genetic stability. Furthermore growth capacity and enzyme characteristics of the mutants were better than those of the parental strains.

Key words: atmospheric and room temperature plasma（ARTP）, Trichoderma harzianum, pesticide resistance, domestication.