西藏藏猪遗传多样性研究

doi:10.13560/j.cnki.biotech.bull.1985.2015.05.034

摘要/Abstract

摘要： 采用mtDNA D-loop作为分子标记，对西藏的4个藏猪群体（林芝、山南、昌都和日喀则）遗传多样性进行了研究。结果表明，西藏藏猪mtDNA D-loop高变区A+T含量（62.90%）明显高于 G+C 含量（37.1%），富含A和T，存在碱基偏倚性。在长度为435 bp的序列中，共检测到20个变异位点，界定了26个单倍型，单倍型多样度（Hd）为0.705±0.021，平均核苷酸差异数（k）为1.231，核苷酸多样度（Pi）为0.002 83。其中，Hd、k和Pi在昌都藏猪群体中最高，日喀则藏猪最低。此外，Hap1和Hap3单倍型是4个群体的共享单倍型，表明4个藏猪群体存在两个共同的母系祖先单倍型。

关键词: 藏猪, mtDNA D-loop, 单倍型, 遗传多样性

Abstract: mtDNA D-loop was selected as a marker to determine genetic diversity of the 4 Tibetan pig populations（Nyingchi, Shannan, Chamdo and Shigatse）in Tibet. The results showed A + T content（62.90%）was significantly higher than the G + C content（37.1%）, indicating that Tibetan pigs’ mtDNA D-loop was rich in A and T; there was the presence of a base bias. A total of 20 variable sites and 26 haplotypes were identified in the 435 bp nucleotide sequence of mtDNA D-loop, haplotype diversity（Hd）was 0.705 ± 0.021, the average number of nucleotide differences（k）was 1.231, and nucleotide diversity（Pi）was 0.00283. Among them, Hd, k and Pi were the highest in Chamdo Tibetan pig population, and lowest in Shigatse. In addition, Hap1 and Hap3 were 4 shared haplotypes, revealing that there were 2 common maternal ancestor haplotypes in 4 Tibetan pig populations.

Key words: Tibetan pig, mtDNA D-loop, haplotype, genetic diversity

郭永博, 蔡原. 西藏藏猪遗传多样性研究[J]. 生物技术通报, 2015, 31(5): 224-230.

Guo Yongbo, Cai Yuan. The Study of Genetic Diversity in Tibetan Pig of Tibet[J]. Biotechnology Bulletin, 2015, 31(5): 224-230.

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