油茶根际溶磷菌的分离、鉴定及溶磷能力研究

doi:10.13560/j.cnki.biotech.bull.1985.2015.07.025

生物技术通报 ›› 2015, Vol. 31 ›› Issue (7): 169-173.doi: 10.13560/j.cnki.biotech.bull.1985.2015.07.025

油茶根际溶磷菌的分离、鉴定及溶磷能力研究

刘小玉, 付登强, 陈良秋, 杨伟波, 李东霞, 符海泉

(中国热带农业科学院椰子研究所油茶研究中心，文昌 571339)

收稿日期:2014-09-04 出版日期:2015-07-16 发布日期:2015-07-16
作者简介:刘小玉，女，研究实习员，硕士研究生，研究方向：土壤微生物与油茶丰产栽培技术；E-mail：liuxiaoyu06120210@163.com
基金资助:
海南省自然科学基金项目(314147)

Isolation，Identification and Phosphate-solubilizing Capacity of Phosphate-solubilizing Bacteria from the Rhizosphere of Camellia

Liu Xiaoyu, Fu Dengqiang, Chen Liangqiu, Yang Weibo, Li Dongxia, Fu Haiquan

(Coconut Research Institute，Oil Camellia Research Center，CATAS，Wenchang 571339)

Received:2014-09-04 Published:2015-07-16 Online:2015-07-16

摘要/Abstract

摘要： 采用传统的微生物分离培养法，对油茶根际溶磷细菌进行了分离，共筛选得到17株溶磷细菌。利用透明圈法对油茶根际土壤中具有溶磷能力的细菌进行初筛；采用钼锑抗比色法测定发酵液的可溶性磷含量，对解磷菌株进行复筛，得出菌株6-Y-09溶磷活性最强。根据进行菌落形态特征、生理生化特征、16S rDNA序列和系统发育分析等研究，初步鉴定菌株 6-Y-09为洋葱伯克霍尔德菌。该菌株在后续微生物菌肥研制中具有较大潜力，为通过生物途径改善油茶磷素供应，促进油茶生长提供了优良的菌株资源。

关键词: 油茶根际, 溶磷菌, 分离和鉴定, 洋葱伯克霍尔德菌

Abstract: Phosphate-solubilizing bacteria were isolated from the rhizosphere of camellia, and 17 strains were screened using traditional method of isolation and culture of microorganisms. By utilizing the method of transparent zone, phosphate-solubilizing bacteria were preliminarily screened from the rhizosphere soil. Subsequently, the bacteria were re-screened by using method of molybdenum-antimony anti-spectrophotometric to measure content of soluble phosphorus in fermentation liquor, and strain 6-Y-09 with the highest capacity of solubilizing phosphate was gained. According to morphology of the colony, physiological and biochemical properties, 16S rDNA sequence and phylogenetic analysis, strain 6-Y-09 was identified as Burkholderia capacia. The strain has great potential in further development of microbiological fertilizer because of its stronger phosphate-solubilizing capability, which will provide an outstanding strain resource for improving phosphorus supply of camellia and promoting its growth.

Key words: camellia rhizosphere, phosphate-solubilizing bacteria, isolation and identification, Burkholderia capacia

刘小玉, 付登强, 陈良秋, 杨伟波, 李东霞, 符海泉. 油茶根际溶磷菌的分离、鉴定及溶磷能力研究[J]. 生物技术通报, 2015, 31(7): 169-173.

Liu Xiaoyu, Fu Dengqiang, Chen Liangqiu, Yang Weibo, Li Dongxia, Fu Haiquan. Isolation，Identification and Phosphate-solubilizing Capacity of Phosphate-solubilizing Bacteria from the Rhizosphere of Camellia[J]. Biotechnology Bulletin, 2015, 31(7): 169-173.

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油茶根际溶磷菌的分离、鉴定及溶磷能力研究

Isolation，Identification and Phosphate-solubilizing Capacity of Phosphate-solubilizing Bacteria from the Rhizosphere of Camellia

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