基因工程和代谢工程在甜菊糖生产上应用进展

doi:10.13560/j.cnki.biotech.bull.1985.2015.09.002

摘要/Abstract

摘要： 甜菊糖是由原产于南美的多年生草本植物甜叶菊所产生的一种糖苷。中国广西一带的甜茶树也产生类似的糖苷。它们作为一种低能量、高甜度的天然甜味剂近几年在欧美、日本及中国得到越来越普遍的利用。中国是世界上最大的甜叶菊种植国。近年来甜菊糖代谢途径中的酶蛋白和有关基因的分离以及甜叶菊转化体系的建立，为通过遗传和代谢工程提高甜菊糖产量和改变甜菊糖苷的组成奠定了基础，也为利用微生物和其他高生物量作物产生甜菊糖提供了新的途径。就甜叶菊的生产、甜菊糖的利用现状以及甜菊糖在植物体内的代谢途径进行了总结。

关键词: 甜叶菊, 甜菊糖, 甜味剂, 代谢工程, 遗传工程

Abstract: Stevia sugar is a unique glycoside produced from stevia(Stevia rebaudiana)of a perennial herb originally growing in South America. Sweet tea tree(Rubus suavissimus), mainly growing in the Guangxi area of China, also produces similar glycoside. In recent years, stevia sugar as a natural high-intensity sweetener has increasingly been used in USA, European countries, Japan, and China. Stevia is extensively cultivated in China. Here we review the current status of stevia production and usage of stevia sugar, as well as progresses on understanding its biosynthetic pathway. Recent advances on identifying the enzymes involved in steviol biosynthesis, isolating relevant genes as well establishing stevia transformation system provide the fundamentals toward improving the production of steviol glycoside and altering the constitute of stevia glycoside through genetic and metabolic engineering, also inspire the novel measures of producing stevia sugar using microorganisms and high biomass crops.

Key words: Stevia rebaudiana, stevia sugar, sweetener, metabolic engineering, genetic engineering

余波颖, 王宁琳, 李国婧, 夏亦荠. 基因工程和代谢工程在甜菊糖生产上应用进展[J]. 生物技术通报, 2015, 31(9): 8-14.

Yu Boying, Wang Ninglin, Li Guojing, Xia Yiji. Application Prospect of Genetic Engmeering and Metabolic Engineering in Improving Its Production of Steviol Glycoside[J]. Biotechnology Bulletin, 2015, 31(9): 8-14.

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