合成生物学开关在代谢工程中的应用

doi:10.13560/j.cnki.biotech.bull.1985.2017.01.006

摘要/Abstract

摘要： 在代谢工程研究领域中合成生物学开关主要用于调控基因的表达。传统的代谢工程改造主要通过敲除和过表达来改变特定基因的表达量。但基因敲除通常会导致菌体生长的下降。因此，我们需要适时的关闭和激活特定基因的表达。合成生物学开关就是解决这一问题的关键工具。目前，在代谢工程中常用的合成生物学开关有光控开关、温度诱导开关、拨动开关和核糖开关。其中，拨动开关和核糖开关在动态调节基因表达上拥有更大优势。介绍了代谢工程中常用的几种合成生物学开关，以及它们在代谢工程的应用。

关键词: 合成生物学, 基因表达调控, 代谢工程, 合成生物学开关, 动态调控

Abstract: Translational switches for synthetic biology are mainly used to regulate gene expression in the field of metabolic engineering research. Traditional metabolic engineering regulates the expression of specific genes by using knockout and overexpression. However,the knockout of genes usually leads to the decrease in growth,therefore,we need to close and activate the expression of specific gene at proper time,and translational switche for synthetic biology is the key tool to solve such issue. At present,light-controlled switch,temperature-induced switch,toggle switch,and riboswitch are commonly used in metabolic engineering,of which the toggle switch and riboswitch present a great advantage in the dynamic regulation of gene expression. In this paper,several kinds of translational switches for synthetic biology and their applications in metabolic engineering are reviewed.

Key words: synthetic biology, gene expression regulation, metabolic engineering, switch for synthesis biology, dynamic regulation

庞庆霄，梁泉峰，祁庆生. 合成生物学开关在代谢工程中的应用[J]. 生物技术通报, 2017, 33(1): 58-64.

PANG Qing-xiao, LIANG Quan-feng, QI Qing-sheng. Application of Switch for Synthetic Biology in Metabolic Engineering[J]. Biotechnology Bulletin, 2017, 33(1): 58-64.

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