生物技术通报 ›› 2015, Vol. 31 ›› Issue (12): 146-149.doi: 10.13560/j.cnki.biotech.bull.1985.2015.12.021

• 研究报告 • 上一篇    下一篇

九香虫醇提物对运动大鼠骨骼肌抗氧化酶活性及其基因表达水平的影响

高颖晖1,2,周万红1,2,窦鹏1,戚一曼1,王敦1   

  1. 1. 西北农林科技大学昆虫研究所,杨凌 712100;
    2. 西北农林科技大学体育部,杨凌 712100
  • 收稿日期:2015-03-30 出版日期:2015-12-19 发布日期:2015-12-19
  • 作者简介:高颖晖,女, 硕士,讲师, 研究方向:运动生理生化;E-mail:yinghuigao@aliyun.com

The Effects of Ethanol Extract from Aspongopus chinensis on the Activities of Antioxidant Enzymes in Skeletal Muscle of Exercised Rats and Their Gene Expression Levels

Gao Yinghui1,2, Zhou Wanhong1,2, Dou Peng1, Qi Yiman1, Wang Dun1   

  1. 1. Institute of Entomology,Northwest Agriculture and Forestry University,Yangling 712100;
    2. P.E. Department,Northwest Agriculture and Forestry University,Yangling 712100
  • Received:2015-03-30 Published:2015-12-19 Online:2015-12-19

摘要:

旨在探讨补充九香虫醇提物对运动大鼠骨骼肌抗氧化酶活性及其基因表达水平的影响。对大负荷游泳训练大鼠补充剂量分别为各组平均体重的0.5、1.0和1.5 g/kg的九香虫提取物,在8周大负荷训练结束后测定骨骼肌抗氧化酶活性与表达水平。8周大负荷训练后,相比单纯大负荷运动组,补充九香虫提取物组SOD、CAT、GST三种抗氧化酶活性均显著增加,同时3种酶的编码基因表达水平也显著上调。说明补充九香虫提取物提高运动大鼠骨骼肌抗氧化酶系活性的机理之一是上调了编码基因的表达水平。

关键词: 九香虫提取物, 运动大鼠, 骨骼肌抗氧化酶, 表达水平, 酶活性

Abstract:

The effects of ethanol extract from Aspongopus chinensis(EEAC)on the activities of antioxidant enzymes in skeletal muscle of exercised rat and their gene expression levels were analyzed in this study. The EEAC was supplemented to rats that were given heavy-load swimming training at 3 dose levels of 0.5 g/kg, 1.0 g/kg and 1.5 g/kg based on the average weight of each group. The activities and encoded genes expression levels of antioxidant enzymes in skeletal muscle were measured after 8 weeks training. The activities of SOD, CAT and GST with EEAC supplementation were increased significantly compared to non-supplemented training rats, and the encoded genes for these 3 enzymes were up-regulated also. The results suggest that the one mechanism for the increase of antioxidant enzymes activities in skeletal muscle of exercised rats is that the expression levels of encoded genes were up-regulated by EEAC supplementation.

Key words: ethanol extract from Aspongopus chinensis, exercised rat, antioxidant enzymes in skeletal muscle, expression level, enzyme activity