内生链霉菌SSD49的抑菌活性和防病促生效果

doi:10.13560/j.cnki.biotech.bull.1985.2016-1121

摘要/Abstract

摘要： 内生链霉菌SSD49分离自羊角拗的根部,对杨树溃疡病病原真菌具有很强的抑菌活性。为了研究该菌对各种病原菌的抑菌活性,促进植物生长的特征和作为生防菌剂的潜力,进行了平板对峙、组织培养和温室盆栽实验。结果显示,SSD49能够抑制板栗疫病菌、大豆核盘菌、辣椒疫霉、苹果轮纹病病原菌、杨树溃疡病细菌型病原菌、以及耐甲氧西林金黄色葡萄球菌和白假丝酵母等多种植物病原菌和人类机会致病菌,并产生几丁质酶和31.56 mg/L的吲哚乙酸;接种SSD49孢子液的毛白杨组培苗、大豆、番茄和辣椒的株高、根长和地上部分干重及根干重分别提高了16.98%-41.82%、19.00%-55.85%、4.58%-70.87%和15.25%-126.06%;对大豆菌核病的防治效果达到88.24%。以上研究结果证明内生链霉菌SSD49在农林业的生物防治中具有潜在的应用价值,生防、促生机理有待进一步深入研究。

关键词: 植物内生菌, 内生链霉菌, 抑菌活性, 生物防治, 植物促生

Abstract: The endophytic Streptomyces sp. SSD49,isolated from the roots of Strophanthus divaricatus,showed strong antifungal activity against Botryosphaeria dothidea. In order to analyze its antimicrobial activity against various pathogens and the characterization of plant-growth-promoting as well as the potential of biocontrol agents,a series of experiments were conducted,including plat confrontation,tissue culture and potting test in greenhouse. The results showed that Streptomyces sp. SSD49 inhibited the bacterial pathogens of Cryphonectria parasitica,Sclerotinia sclerotiorum,Phytophthora capsici,Dothiorella gregaria,Lonsdalea quercina subsp. populi,as well as plant pathogens and human opportunistic pathogens of Methicillin-resistant Staphylococcus aureus and Candida albicans. It secreted chitinase and produced indole acetic acid at the concentration of 31.56 mg/L. The shoot height,root length,shoot and root dry weight of Populus tomentosa tissue seedlings,soybean,tomato and pepper plants inoculated with strain SSD49 increased by 16.98-41.82%,19.00-55.85%,4.58-70.87% and 15.25-126.06%,respectively. The inhibition rate to Sclerotinia sclerotiorum reached 88.24%. The above results demonstrated that endophytic Streptomyces sp. SSD49 is potentially applicable in controlling plant disease in agriculture and forestry,while the mechanism of plant disease control and plant-growth-promoting needs to be further studied.

Key words: endophyte, endophytic Streptomyces, antimicrobial activities, plant disease control, plant-growth-promoting

葛优优, 刘晓瑜, 窦桂铭, 马玉超. 内生链霉菌SSD49的抑菌活性和防病促生效果[J]. 生物技术通报, 2017, 33(6): 121-127.

GE You-you, LIU Xiao-yu, DOU Gui-ming, MA Yu-chao. Antimicrobial Activities and Efficacy of Endophytic Streptomyces sp. SSD49 in Plant Disease Control and Plant-growth-promoting[J]. Biotechnology Bulletin, 2017, 33(6): 121-127.

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