微生物浸出过程中胞外蛋白的作用及其机理研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2016-1197

摘要/Abstract

摘要： 矿物-微生物-溶液多相界面是硫化矿生物浸出的关键场所，界面微生物胞外多聚物（EPS）关键组分胞外蛋白在生物膜形成、结构稳定和硫化矿溶解等方面起到关键作用。综述了生物浸出过程中EPS关键组分胞外蛋白的研究进展和胞外蛋白现有的研究方法及其对冶金微生物胞外蛋白研究的适用性，展望了浸矿微生物胞外蛋白研究的前景，旨为生物冶金领域研究EPS关键组分胞外蛋白结构及其作用机理提供重要的理论和方法支撑。

关键词: 生物浸出, 胞外多聚物（EPS）, 胞外蛋白, 激光共聚焦显微镜（CLSM）

Abstract: Mineral-microbe-solute multi phase interface is the key space for bioleaching of sulfide minerals. Microbial proteins at the interface as the key components of extracellular polymeric substances（EPS）play a key role in the biofilm formation，structure maintainance and sulfide mineral dissolution. This article reviews the developments，existing methods and their applicability for studying the extracellular proteins in EPS of metallurgical microorganisms during bioleaching，and prospects a vista of future research on the extracellular protein produced by bioleaching microorganisms. These will offer important theoretical basis for studying the structure and functional mechanisms of extracellular proteins in the bioleaching process.

Key words: bioleaching, extracellular polymeric substances, extracellular proteins, confocal laser scanning microscopy

余润兰,刘阿娟,刘亚楠,刘学端,邱冠周,曾伟民. 微生物浸出过程中胞外蛋白的作用及其机理研究进展[J]. 生物技术通报, 2017, 33(7): 62-68.

YU Run-lan ,LIU A-juan ,LIU Ya-nan, LIU Xue-duan, QIU Guan-zhou, ZENG Wei-min. The Research Progress of the Functions and Mechanism of Extracellular Proteins in Bioleaching[J]. Biotechnology Bulletin, 2017, 33(7): 62-68.

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