核酸等温扩增技术在微生物快速检测中的研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2017-0106

摘要/Abstract

摘要： 等温扩增技术在近20多年，得到了快速发展，依靠其等温反应的优势正逐渐取代传统的体外扩增技术聚合酶链式反应（Ploymerase chain reaction，PCR），其已经在临床医学、检验医学、分子生物学、基因组学、食品安全等不同领域中的发挥着重要作用，特别是在致病菌、病毒等微生物的检测中。将选取10余种关注度较高的等温扩增技术，主要针对其反应原理、优缺点以及发展应用等方面进行简要概述和对比，最后展望了等温扩增技术未来的发展趋势，以期对相关研究领域的发展起到积极的促进作用。

关键词: 等温扩增, 微生物, 核酸, 快速检测

Abstract: In recent 20 years，isothermal amplification technology has been rapidly developed. Relying on the advantage of the isothermal reaction，it is gradually replacing the traditional in vitro PCR（polymerase chain reaction）amplification，and plays an important role in different fields such as clinical medicine，laboratory medicine，molecular biology，genomics，food safety，and especially in the detection of pathogenic bacteria，viruses and other microorganisms. This article reviews and compares more than 10 kinds of high-profile isothermal amplification technologies，mainly focusing on the reaction principles，advantages，disadvantages as well as the development and application，and finally prospects the trends of isothermal amplification techniques，in order to play a positive role in the development of the related research fields.

Key words: isothermal amplification, microorganisms, nucleic acid, rapid detection?

王大洲,郭天笑,郑实,商颖,许文涛. 核酸等温扩增技术在微生物快速检测中的研究进展[J]. 生物技术通报, 2017, 33(7): 49-61.

WANG Da-zhou ,GUO Tian-xiao ,ZHENG Shi, SHANG Ying, XU Wen-tao. Research Progress on the Isothermal Nucleic Acid Amplification Techniques in Rapid Detection of Microorganisms[J]. Biotechnology Bulletin, 2017, 33(7): 49-61.

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