生物技术通报 ›› 2016, Vol. 32 ›› Issue (2): 146-151.doi: 10.13560/j.cnki.biotech.bull.1985.2016.02.019

• 研究报告 • 上一篇    下一篇

一株新的染料甲基红降解菌株 Paracoccus sp.L-4的分离、鉴定及其脱色特性研究

陈亮, 王莉   

  1. 安徽建筑大学环境与能源工程学院,合肥 230601
  • 收稿日期:2015-04-09 出版日期:2016-02-24 发布日期:2016-02-25
  • 作者简介:陈亮,男,硕士研究生,研究方向:偶氮类染料的微生物降解;E-mail:1028383665@qq.com
  • 基金资助:
    国家自然科学基金项目(21107002)

Isolation, Identification and Decolonization Characteristics of a New Methyl Red Degrading Bacterial Strain Paracoccus sp.L-4

CHEN Liang, WANG Li   

  1. (College of Environment and Energy Engineering,Anhui University of Architecture,Hefei 230601)
  • Received:2015-04-09 Published:2016-02-24 Online:2016-02-25

摘要: 从副球菌属首次分离到一株有较强甲基红降解能力的菌株Paracoccus sp.L-4,在LB培养基中,16 h内使100 mg/L培养液甲基红降解掉91.74%,2 d 内可使甲基红降解率近达100%。该菌株降解甲基红适宜的温度和pH值范围分别为25-30℃、pH6.0-7.0,Zn2+、Co2+、Cu2+、Ag+、Al3+、Fe3+、Fe2+ 对甲基红的降解有明显抑制作用。菌株L-4在好氧和缺氧条件均能较好降解甲基红,装液量≤100 mL时脱色率随装液量增加而减少,装液量≥125 mL时,12 h时降解率仍大于40%。菌株L-4在染料废水的生物处理方面有一定的应用价值。

关键词: 甲基红, Paracoccus sp.L-4, 分离, 鉴定, 降解特性

Abstract: The strain Paracoccus sp.L-4 was isolated from Paracoccussp.at first time, and it had the strong ability to degrade methyl red.In LB medium, methyl red in the concentration of 100 mg/L was degraded 91.74% in 16 h, and approximately 100% in 2 d.Furthermore, the optimal conditions for degrading methyl red were 25-30℃ and pH6.0-7.0, respectively.And Zn2+, Co2+, Cu2+, Ag+, Al3+, Fe3+ andFe2+ had an significant inhibitory effects on the degradation of methyl red.The degradation and decolorization of methyl red by strain L-4 was performed well in both aerobic and anoxic conditions.When the volume of liquid was ≤ 100 mL, the ratio of decolorization decreased with the increase of volume of liquid.However, when the amount of liquid was ≥ 125 mL, the degradationrate of methyl red was still over 40% at 12 h, indicating that strain L-4 hasa potential application value inbiological treatmentof dye wastewater.

Key words: methyl red, Paracoccus sp.L-4, isolation, identification, degradation characteristics