高富硒酵母的筛选及富硒强化研究

doi:10.13560/j.cnki.biotech.bull.1985.2017-0604

生物技术通报 ›› 2017, Vol. 33 ›› Issue (12): 132-137.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0604

高富硒酵母的筛选及富硒强化研究

娄兴丹¹, 张根林¹, 叶邦策^1,2

1. 石河子大学化学化工学院新疆兵团化工绿色过程重点实验室，石河子 832003;
2. 华东理工大学，上海 200237

收稿日期:2017-07-21 出版日期:2017-12-25 发布日期:2017-12-21
作者简介:娄兴丹，女，硕士研究生，研究方向：微生物工程；E-mail：1009502440@qq.com
基金资助:
新疆兵团科技攻关计划（2016AB009）

Screening of High Selenium-enriched Yeast and Enhancement of Its Selenium-enriched Capacity

LOU Xing-dan¹, ZHANG Gen-lin¹, YE Bang-ce^{1, 2}

1. School of Chemistry and Chemical Engineering，Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan，Shihezi University，Shihez 832003;
2. Shanghai East China University of Science and Technology，Shanghai 200237

Received:2017-07-21 Published:2017-12-25 Online:2017-12-21

摘要/Abstract

摘要： 富硒酵母在医学保健和制药产业中有着重要的应用，高富硒酵母是富硒酵母产品开发的基础。以新疆富硒土壤为样品来源，通过马丁培养基的初筛和富硒能力的复筛，筛选到一株富硒量相对较高的黏红酵母菌株Rhodotorula glutinis X-20。优化了菌株的富硒培养条件，结果显示，利用2%甘油为碳源可显著提高酵母中硒的累积，培养12 h时添加50 μg/mL的亚硒酸钠能够保证酵母较高的硒累积量和生物量，添加0.4 mmol/L的磷酸盐可以有效地促进硒的转运和累积，最终使Rhodotorula glutinis X-20富硒量达到5 009 μg/g。

关键词: 富硒土壤, 黏红酵母, 筛选, 优化培养

Abstract: Selenium yeast is important for health and pharmaceutical industry，and high selenium-enriched yeast is the basis for the development of selenium-enriched yeast products. In this study，Rhodotorula glutinis X-20 with relatively high amount of enriched selenium was screened from selenium-enriched soil of Xinjiang via preliminary screening with Martin medium and re-screening of selenium-enriching capacity. The optimal culture conditions were determined through single experimental factors. The selenium content enriched by the yeast was significantly improved by using 2% glycerol as carbon source. Supplying sodium selenite at 12 h of cultivation ensured the higher selenium content and biomass of the yeast. Addition of 0.4 mM phosphate efficiently increased the transport and accumulation of selenium，and ultimately the enriched selenium content by strain X-20 reached 5009 μg/g.

Key words: selenium-enriched soil, Rhodotorula glutinis, screen, optimized culture

娄兴丹, 张根林, 叶邦策，. 高富硒酵母的筛选及富硒强化研究[J]. 生物技术通报, 2017, 33(12): 132-137.

LOU Xing-dan, ZHANG Gen-lin, YE Bang-ce. Screening of High Selenium-enriched Yeast and Enhancement of Its Selenium-enriched Capacity[J]. Biotechnology Bulletin, 2017, 33(12): 132-137.

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高富硒酵母的筛选及富硒强化研究

Screening of High Selenium-enriched Yeast and Enhancement of Its Selenium-enriched Capacity

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