一株四环素高效降解菌的分离及降解特性

doi:10.13560/j.cnki.biotech.bull.1985.2017-0723

生物技术通报 ›› 2018, Vol. 34 ›› Issue (5): 172-178.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0723

一株四环素高效降解菌的分离及降解特性

吴学玲^1,2,吴晓燕¹,李交昆^1,2,申丽^1,2,余润兰^1,2,曾伟民^1,2

1. 中南大学资源加工与生物工程学院,长沙 410083;
2. 中南大学生物冶金教育部重点实验室,长沙 410083

收稿日期:2017-09-10 出版日期:2018-05-26 发布日期:2018-06-07
作者简介:吴学玲,女,博士,研究方向：环境微生物学;E-mail：wxlcsu@csu.edu.cn
基金资助:
国家自然科学基金面上项目（31470230）,国家自然科学基金国际合作重点项目（51320105006）,中南大学研究生自主探索创新项目（3018zzts767）

Isolation and Degradation Characteristics of a Efficient Tetracycline-degrading Strain

WU Xue-ling^{1, 2}, WU Xiao-yan¹, LI Jiao-kun^{1, 2}, SHEN Li^{1, 2}, YU Run-lan^{1, 2}, ZENG Wei-min^{1, 2}

1. School of Minerals Processing and Bioengineering,Central South University,Changsha 410083;
2. Key Laboratory of Biometallurgy of Ministry of Education,Central South University,Changsha 410083

Received:2017-09-10 Published:2018-05-26 Online:2018-06-07

摘要/Abstract

摘要： 四环素类抗生素在养殖业中广泛使用引起药物残留,给环境及人类健康带来了深远影响。从利用四环素类抗生素作为饲料添加剂的养猪厂底泥中,筛选获得一株能够高效降解四环素的细菌XY-1,经革兰氏染色、形态学观察、生理生化及16S rDNA序列分析等,确定该菌株属于拉乌尔菌属,命名为Raoultella sp.XY-1。采用高效液相色谱（HPLC）测定了菌株XY-1对四环素的降解能力,结果表明,该菌株最佳降解条件是温度为25℃,初始pH值为7.0,在此最佳条件下,XY-1在第8天时生长量最大且四环素降解率最高达到70.68%。对XY-1的四环素耐药基因进行了初步探究,首次在该菌株中检测到两种四环素耐药基因tet（D）和tet（E）。

关键词: 四环素降解菌, 筛选及鉴定, 降解特性, 四环素耐药基因

Abstract: The wide use of tetracycline antibiotics in aquaculture causes drug residues,which brings serious impacts to environment and human health. In this study,a strain XY-1,which efficiently degraded tetracycline,was screened from the sediment of pig farm using tetracycline antibiotics as feed additive. After the Gram stain,morphological observation,physiological and biochemical and 16S rDNA sequence analysis,the strain was identified as Raoultella sp. and named as XY-1.The ability of strain degrading tetracycline was determined by high performance liquid chromatography（HPLC）. The results showed that the optimal degradation conditions were as followings：the temperature was 25℃ and the initial pH was 7.0. Under the optimal conditions,XY-1 grew at most and the highest tetracycline degradation rate reached 70.68% at the 8th day. Besides,the preliminary probe on the tetracycline-resistant genes was also conducted,and it was the first time to have two tetracycline-resistant genes tet（D）and tet（E）detected in the strain.

Key words: tetracycline-degrading strain, screening and identification, degradation characteristics, tetracycline-resistance gene

吴学玲,吴晓燕,李交昆,申丽,余润兰,曾伟民. 一株四环素高效降解菌的分离及降解特性[J]. 生物技术通报, 2018, 34(5): 172-178.

WU Xue-ling, WU Xiao-yan, LI Jiao-kun, SHEN Li, YU Run-lan, ZENG Wei-min. Isolation and Degradation Characteristics of a Efficient Tetracycline-degrading Strain[J]. Biotechnology Bulletin, 2018, 34(5): 172-178.

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一株四环素高效降解菌的分离及降解特性

Isolation and Degradation Characteristics of a Efficient Tetracycline-degrading Strain

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