关键词: 四环素降解菌, 筛选, 鉴定, 降解性能

Abstract: Tetracycline antibiotics have been widely used in the livestock industry, but its complex structure, difficult to degrade, easy to accumulate in the water environment and produce a far-reaching impact on the ecological environment, antibiotic pollution as an important environmental issues has expanded the related basic research. Microbial degradation of the environment antibiotic pollution in recent years become a hotspot method. The selective media was used to isolate and screen the tetracycline-degrading bacteria. A high efficiency tetracycline-degrading bacterium（4002）was selected from sewage of a pharmaceutical factory. According to the morphological features, physiological and biochemical characteristics and the sequence analysis of 16S rDNA, the strain was identified as Advenella kashmirensi. Besides, the pH, dissolved oxygen, inorganic salts and other aspects of the strains were analyzied to study the degradation performance of tetracycline. The results showed that, the strain suitable conditions for the degradation of tetracycline are pH7.0, under aerobic condition, 150 r/min and cultured with shaking 6 d, the initial concentration of 50 μg/mL tetracycline degradation rate of 57.8%. FeSO₄ and MnSO₄ can promote degradation of tetracycline and little impact on MgSO₄, but CaSO₄ can inhibit degradation. After cultured for 8 d, the culture solution by HPLC detection showed at 6.022 min emergence of a new absorption peak, which was speculated as the degradation product.

Key words: tetracycline-degrading strain, screening, identification, degradation performance