链霉菌30702的鉴定及其生防特性

doi:10.13560/j.cnki.biotech.bull.1985.2017-1083

摘要/Abstract

摘要： 对从海南粗榧根际分离的一株链霉菌菌株30702进行菌种鉴定和生物防治特性的研究。利用16S rRNA基因序列和多位点序列分析（MLSA），并结合形态和生理生化特征比较分析确定该菌株种水平的分类地位。该菌株鉴定为紫黑链霉菌进化分枝中的Streptomyces solisilvae；该菌株能够产生蛋白酶、纤维素酶、几丁质酶、β-葡聚糖酶、1-氨基环丙烷-1-羧酸（ACC）脱氨酶、铁载体，具有解磷活性和促进植物生长的特性，发酵代谢产物能够抑制山药炭疽菌孢子萌发和菌丝生长。菌株30702具有优良的生防特性，在植物病害的生物防治上具有潜在的开发利用价值。

关键词: 链霉菌, 紫黑链霉菌进化分枝, 生防特性

Abstract: This work is to identify a strain of Streptomyces sp. 30702 isolated from rhizosphere of Cephalotaxus hainanensis and to estimate its biocontrol traits. According to analysis of the 16S rRNA gene sequences and multilocus sequence analysis（MLSA）as well as comparison of physiological and morphological characteristics，the Streptomyces sp. 30702 was identified as Streptomyces solisilvae，a member of the Streptomyces violaceusniger clade. The strain was able to produce protease，cellulase，chitinase，β-glucanase，1-aminocyclopropane-1-carboxylic acid（ACC）deaminase，and siderophore，and also had traits of plant growth-promoting and phosphate solubilization. Additionally，the fermented extract of strain 30702 inhibited the spore germination and mycelial growth of Colletotrichum gloeosporioides（anthracnose of yam）. The results indicate that the Streptomyces strain 30702 possesses promising biocontrol characteristics, thus it has potential development and application values in the biocontrol of plant diseases.

Key words: Streptomyces, Streptomyces violaceusniger clade, biocontrol characteristics

丛子文 ,焦敬华, 周双清 ,黄东益 ,吴文嫱 ,许云 ,夏薇 ,张荣萍 ,黄小龙. 链霉菌30702的鉴定及其生防特性[J]. 生物技术通报, 2018, 34(6): 190-198.

CONG Zi-wen ,JIAO Jing-hua ,ZHOU Shuang-qing, HUANG Dong-yi ,WU Wen-qiang ,XU Yun ,XIA We,i ZHANG Rong-ping ,HUANG Xiao-long. Identification and Biocontrol Characteristics of Streptomyces sp. 30702[J]. Biotechnology Bulletin, 2018, 34(6): 190-198.

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