植物根际促生细菌定殖研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2017.02.002

摘要/Abstract

摘要： 植物促生菌（plant grwth-promoting bacterium,PGPB）因可有效抑制根际病原菌,促进植物生长,增加作物产量等作用,在农业生产领域展现出巨大前景。以了解植物根际促生细菌的种类,理解定殖相关的促生机制为目的,展示了根际细菌的定殖过程及影响细菌在根部定殖的因素,通过抗生素标记、免疫学方法、外源基因标记等方法进行定殖微生物的检测,以高通量测序技术在根际定殖中的广泛应用为结果,得出结论应从遗传水平上对菌株进行解读,获得植物根际土壤微生物群落多样性,功能基因等研究,这项工作对预测根际促生细菌与植物的交互作用,生物菌剂的田间应用有重要意义,应是植物根际促生细菌今后的研究方向。

关键词: 定殖, 根际菌, 促生, 高通量测序

Abstract: The PGPB（plant growth-promoting bacterium）presents a promising prospect in agriculture due to its advantages such as inhibiting rhizosphere pathogens,promoting plant growth,and increasing crop production. This paper first summarizes the species of plant growth-promoting rhizobacteria and the promoting mechanisms of involved colonization,and demonstrates the colonization process of rhizobacteria as well as the factors influencing the colonization of bacteria in rhizosphere. Then the paper reviews the detection methods of microorganisms in the colonization of bacteria,including antibiotics marking,immunological methods,and exogenous gene marking methods. Further,the paper discusses high-throughput sequencing technologies widely used in the rhizosphere colonization. Finally,it is concluded that the future researches on interpreting strains in genetic level,gaining microbial community diversity in plant rhizosphere and the functional genes should be of significance to better predict the interaction of PGPB and its host plant,and to apply bio-fertilizers in field.

Key words: colonization, rhizobacteria, growth-promoting, high-throughput sequencing

孙真, 郑亮, 邱浩斌. 植物根际促生细菌定殖研究进展[J]. 生物技术通报, 2017, 33(2): 8-15.

SUN Zhen, ZHENG Liang, QIU Hao-bin. Research Advances on Colonization of Plant Growth-promoting Rhizobacteria[J]. Biotechnology Bulletin, 2017, 33(2): 8-15.

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