外切酶III介导的功能核酸生物传感器研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2018-0278

摘要/Abstract

摘要：

外切酶III（Exonuclease III,Exo III）是一种可以特异性识别并催化dsDNA从3'-羟基端逐步水解并释放出单核苷酸的核酸酶,而对ssDNA的水解作用十分有限。基于Exo III的外切酶活性,搭载不同的信号输出方式,该酶已被成功运用到DNA、小分子和金属离子等靶物质的放大检测中。本文主要根据所检测的靶物质不同,对Exo III介导的生物传感器进行了分类综述,阐述了各传感器的基本设计原理和灵敏度等方面内容。此外,对Exo III与其他信号放大策略或纳米材料相结合的最新研究进展也做了较为详细的介绍。最后,对Exo III介导的生物传感器的优缺点和发展前景进行了总结和展望,有助于Exo III介导的生物传感器在环境监管、生物分析或临床诊断领域的深层次应用和继续研发。

关键词: 外切酶III, 功能核酸, 信号放大, 生物传感器

Abstract:

Exonuclease III（Exo III）is a kind of nuclease that can specifically recognize dsDNA and catalyze the release of mononucleotides by stepwise hydrolysis from 3'-hydroxyl termini of dsDNA strands;while hydrolysis on ssDNA is limited. Based on its exonuclease activity,Exo III has been successfully employed for amplified detection of DNAs,small molecules and metal ions while combined with a series of signal outputs. Here we mainly give a detailed classifying description of the Exo III-assisted biosensors based on various target substances,and also expound the working principles and sensitivity of each sensor. In addition,we discuss the recent progresses on the integration of Exo III-assisted target-recycling strategy with other signal enhancement strategies or emerging nanomaterials in detail as well. Finally,we summarize the advantages and disadvantages of the Exo III-assisted biosensors and prospect the future development,which will be conducive to further application and continuous development of Exo III-assisted biosensors in environmental management,biological analysis and clinical diagnosis.

Key words: exonuclease III, functional nucleic acid, signal amplification, biosensor

宋欢, 罗云波, 许文涛. 外切酶III介导的功能核酸生物传感器研究进展[J]. 生物技术通报, 2018, 34(9): 55-69.

SONG Huan, LUO Yun-bo, XU Wen-tao. Research Progress on Exonuclease III-assisted Functional Nucleic Acid Biosensors[J]. Biotechnology Bulletin, 2018, 34(9): 55-69.

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