苏云金芽胞杆菌G03菌株的比较基因组分析

doi:10.13560/j.cnki.biotech.bull.1985.2018-0859

生物技术通报 ›› 2018, Vol. 34 ›› Issue (10): 187-193.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0859

苏云金芽胞杆菌G03菌株的比较基因组分析

姚萌^{1, 2}, 王奎^{1, 2}, 束长龙², 杜立新³, 李海涛¹, 丁明月¹, 刘荣梅¹

1. 东北农业大学生命科学学院,哈尔滨 150030;
2. 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京 100193;
3. 河北农林科学院植物保护研究所,保定 071000

收稿日期:2018-09-30 出版日期:2018-10-26 发布日期:2018-11-07
作者简介:姚萌,女,硕士,研究方向：生防微生物;E-mail：234388435@qq.com
基金资助:
国家重点研发计划子课题（2017YFD0201201）,黑龙江省科学基金面上项目（C2016025）

Comparative Genomic Analysis of Bacillus thuringiensis Strain G03

YAO Meng^{1, 2}, WANG Kui^{1, 2}, SHU Chang-long², DU Li-xin³, LI Hai-tao¹, DING Ming-yue¹, LIU Rong-mei¹

1. College of Life Sciences,Northeast Agricultural University,Harbin 150030;
2. State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193;
3. Institute of Plant Protection,Hebei Academy of Agricultural and Forestry Sciences,Baoding 071000

Received:2018-09-30 Published:2018-10-26 Online:2018-11-07

摘要/Abstract

摘要： 苏云金芽胞杆菌（Bacillus thuringiensis,Bt）对包括鳞翅目、鞘翅目在内的多种昆虫具有特异高效的毒杀活性,并且对非靶标生物安全,是目前研究最多、应用最广的生防微生物。G03是一株对鳞翅目害虫高效的Bt菌株,属于鲇泽亚种,目前已经用于害虫防治。为了进一步分析其与目前市场上防治鳞翅目害虫用的较多的Btk（B. thuringiensis subsp. kurstaki）菌株HD1之间的差别,明确其遗传学特点,本研究测定了G03基因组,与其他实验室常用菌株进行了基因组比较,重点分析了杀虫相关基因的特点。分析结果显示,G03菌株含有HD1不具有的cry1Ca、cry1Da基因,使其在虫害控制方面更有优势;与HD1相比,G03含有较少的噬菌体、转座子相关基因,这显示G03基因组可能有更好的遗传稳定性,使其在发酵中更稳定。相关研究结果对今后该菌株改良及应用都具有重要的指导意义。

关键词: 苏云金芽胞杆菌, 杀虫剂, 基因组测序, 比较基因组

姚萌, 王奎, 束长龙, 杜立新, 李海涛, 丁明月, 刘荣梅. 苏云金芽胞杆菌G03菌株的比较基因组分析[J]. 生物技术通报, 2018, 34(10): 187-193.

YAO Meng, WANG Kui, SHU Chang-long, DU Li-xin, LI Hai-tao, DING Ming-yue, LIU Rong-mei. Comparative Genomic Analysis of Bacillus thuringiensis Strain G03[J]. Biotechnology Bulletin, 2018, 34(10): 187-193.

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苏云金芽胞杆菌G03菌株的比较基因组分析

Comparative Genomic Analysis of Bacillus thuringiensis Strain G03

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