纳米TiO2和四环素暴露对耐药大肠杆菌的影响

doi:10.13560/j.cnki.biotech.bull.1985.2019-0528

摘要/Abstract

摘要： 以耐药大肠杆菌（M13）和敏感质控菌株（E.coli ATCC25922）为研究对象,探究纳米TiO₂和四环素对大肠杆菌生长的影响。结果表明,与单独添加梯度浓度四环素相比,纳米TiO₂的加入进一步抑制了敏感菌的生长,而耐药菌变化不大。纳米TiO₂单独和联合暴露下对耐药菌的抑菌率均在40%以下,而敏感菌在80%-100%之间,耐药菌M13除了耐抗生素,对纳米TiO₂也存在抗性。微生物对抗生素和其它环境毒性因子的抗性机制存在着一些共性,但在共暴露条件下微生物的抗性机制还有待进一步研究。

关键词: 耐药大肠杆菌, 纳米二氧化钛, 四环素, 抑菌率

Abstract: The drug-resistant Escherichia coli（M13）and sensitive quality control strain（E. coli ATCC25922）were used to study the effects of nano-TiO₂ and tetracycline on the growth of E. coli. The results showed that the addition of nano-TiO₂further inhibited the growth of sensitive bacteria compared with the addition of the gradient concentration of tetracycline alone,while the drug-resistant bacteria did not change much. The antibacterial rate of nano-TiO₂ alone or co-exposure to the drug-resistant bacteria was below 40%,while that to the sensitive bacteria ranged in 80% and 100%. The drug-resistant strain M13 was resistant to antibiotics and also to nano-TiO₂.There are some commonalities between microbial resistance mechanisms against antibiotics and other environmental virulence factors,but the mechanism of microbial resistance under co-exposure conditions remains to be further studied.

Key words: drug-resistant Escherichia coli, nano-TiO₂, tetracycline, antibacterial rate

张永丽, 袁伟, 杨清香. 纳米TiO₂和四环素暴露对耐药大肠杆菌的影响[J]. 生物技术通报, 2019, 35(11): 124-131.

ZHANG Yong-li, YUAN Wei, YANG Qing-xiang. Effects of Nano-TiO₂ and Tetracycline Exposure on Drug-resistant Escherichia coli[J]. Biotechnology Bulletin, 2019, 35(11): 124-131.

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纳米TiO₂和四环素暴露对耐药大肠杆菌的影响

Effects of Nano-TiO₂ and Tetracycline Exposure on Drug-resistant Escherichia coli

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