镧对铜胁迫下水稻转录组模式的调控分析

doi:10.13560/j.cnki.biotech.bull.1985.2020-0110

摘要/Abstract

摘要： 探究稀土镧对铜胁迫下水稻转录组的影响,鉴定镧在调控水稻铜胁迫应答中的关键基因和功能。利用抗氧化酶活性筛选最适铜胁迫及镧处理浓度,进行转录组测序和差异表达分析,并用qRT-PCR验证差异表达水平。对测序数据进行差异表达分析发现3 222个基因显著上调,3 798个显著下调,qRT-PCR验证了4个细胞壁防御相关基因CHIT13、Laccase、Expansin和GH3.4。功能和通路分析获得95组GO条目和112条KEGG通路,富集于激酶和氧分子结合等分子功能、细胞壁及质膜等细胞组分、次级代谢和脂质代谢等生物学过程以及苯丙烷生物合成和植物激素信号转导通路。镧通过调节细胞壁形成和组分提高水稻铜胁迫耐受性。

关键词: 水稻, 转录组, 铜胁迫, 镧, 测序

Abstract: This work is to investigate the effects of rare earth lanthanum on rice transcriptome under copper stress,and to identify key genes and functions of lanthanum-regulated responses in rice against copper stress. The optimal copper stress and lanthanum treatment concentrations were screened by antioxidant enzyme activity,transcriptome sequencing and differential expression analysis were performed,and differential expression levels were verified by qRT-PCR. Differential expression analysis of sequencing data revealed that 3 222 genes were significantly up-regulated and 3 798 genes were significantly down-regulated. Four cell wall defense-related genes CHIT13,Laccase,Expansin,and GH3.4 were verified by qRT-PCR. Total 95 Gene Ontology terms and 112 KEGG pathways were obtained by function and pathway enrichment analysis,and they enriched in molecular functions such as kinase and oxygen molecule binding,cell components such as cell wall and plasma membrane,biological processes such as secondary metabolism and lipid metabolism,as well as pathways e.g. phenylpropane biosynthesis,and plant hormone signal transduction. The molecular mechanism is that La increases the tolerance of rice to copper stress by regulating cell wall formation and components.

Key words: rice, transcriptome, copper stress, lanthanum, sequencing

仲雨晴, 陈佳佳. 镧对铜胁迫下水稻转录组模式的调控分析[J]. 生物技术通报, 2020, 36(8): 8-14.

ZHONG Yu-qing, CHEN Jia-jia. Regulation of Lanthanum on Rice Transcriptome Patterns Under Copper Stress[J]. Biotechnology Bulletin, 2020, 36(8): 8-14.

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