表面活性剂对琼式不动杆菌LH-1-1降解溴氰菊酯的影响

doi:10.13560/j.cnki.biotech.bull.1985.2020-0674

摘要/Abstract

摘要：

旨在探究表面活性剂对菌株降解溴氰菊酯（Deltamethrin,DM）的影响。以前期筛选到具备DM较高降解能力的Acinetobacter junii LH-1-1为目标菌株,通过荧光光谱法测定了3种不同类型表面活性剂（CTAB、AES和Tween-20）的临界胶束浓度（Critical Micelle Concentration,CMC）,并探究不同表面活性剂各CMC对A. junii LH-1-1生长、DM增溶作用和菌株降解DM的影响。结果显示,测得CTAB、AES、Tween-20的CMC分别为0.793 mmol/L、0.547 mmol/L和0.031 mmol/L;CTAB对菌株的生长无明显影响,AES对菌株生长有抑制作用,Tween-20对菌株的生长有一定的促进作用;CTAB、AES和Tween-20对DM均具有较强的增溶作用;且CTAB可显著提高A. junii LH-1-1对DM的降解率,当其含量为2 CMC,72 h时DM降解率达到98.20%,较未添加表面活性剂的对照组（阳性）降解率提高了24.31%。阳离子表面活性剂CTAB可显著提高A. junii LH-1-1对DM的降解。

关键词: 表面活性剂, 琼式不动杆菌LH-1-1, 溴氰菊酯, 生物降解

Abstract:

It is aimed to investigate the effects of surfactants on the degradation of deltamethrin（DM）by strain. The previous screened Acinetobacter junii strain LH-1-1,which has a high degradability on DM,was selected as target strain. The critical micelle concentration（CMC）of three different types of surfactants（CTAB,AES,and Tween-20）werefirst determined by fluorescence spectrometry,and then the effects of the CMCs of CTAB,AES,and Tween-20 on the strain’s growth,and solubility and degradation of DM by the strain were investigated. The CMC of CTAB,AES,and Tween-20 was 0.793 mmol/L,0.547 mmol/L,and 0.031 mmol/L,respectively. The results showed that the strain growth was not significantly affected by using CTAB,while inhibited by AES,and Tween-20 had a certain promotion on the strain’s growth. The solubility of DM was significantly improved by using CTAB,AES and Tween-20. The degradation rate of DM was significantly enhanced by A. junii LH-1-1 in the presence of CTAB. When the concentration of CTAB was 2 CMC,the degradation rate of DM was 98.20% within 72 h,which was 24.31% higher than the positive control group（without the addition of surfactants）. The cationic surfactant CTAB may significantly improve the degradation of DM by A. junii LH-1-1.

Key words: surfactant, Acinetobacter junii LH-1-1, deltamethrin, biodegradation

吴敏, 唐洁, 胡琼, 雷丹, 张庆. 表面活性剂对琼式不动杆菌LH-1-1降解溴氰菊酯的影响[J]. 生物技术通报, 2021, 37(1): 215-222.

WU Min, TANG Jie, HU Qiong, LEI Dan, ZHANG Qing. Effects of Surfactants on Deltamethrin Degradation by Acinetobacter junii LH-1-1[J]. Biotechnology Bulletin, 2021, 37(1): 215-222.

图/表 5

图1 芘在不同浓度表面活性剂下的荧光光谱 A：CTAB;B：AES;C：Tween-20

图2 I1/I3随-lnc（底物）变化曲线 A：CTAB;B：AES;C：Tween-20

图3 表面活性剂对菌株LH-1-1生长的影响 A：CTAB;B：AES;C：Tween-20

图4 表面活性剂对DM的增溶作用

图5 表面活性剂对DM降解的影响

参考文献 39

[1]	Wu YQ, Li WH, Yuan MR, et al. The synthetic pyrethroid deltamethrin impairs zebrafish(Danio rerio)swim bladder development[J]. Sci Total Environ, 2020,701:134870. URL pmid: 31726413
[2]	张小俊, 陆宏达, 田全全, 等. 溴氰菊酯对中华绒螯蟹的毒性作用和组织病理研究[J]. 生态毒理学报, 2018(6):342-351.
	Zhang XJ, Lu HD, Tian QQ, et al. Effects of deltamethrin on histopathology of Chinese Mitten Crab Eriocheir sinensis[J]. Asian Journal of Ecotoxicology, 2018,13(6):342-351.
[3]	洪宇航, 黄毅. 溴氰菊酯对中华绒螯蟹血细胞DNA的损伤[J]. 水产科学, 2018,37(4):544-549.
	Hong YH, Huang Y. Effects of deltamethrin on DNA damage in hemocytes of Chinese Mitten Handed Crab, Eriocheir sinensis[J]. Fisheries Science, 2018,37(4):544-549.
[4]	苏志俊, 刘永涛, 徐春娟, 等. 一株溴氰菊酯降解菌的分离鉴定及降解特性研究[J]. 淡水渔业, 2019,49(1):98-106.
	Su ZJ, Liu YT, Xu CJ, et al. Isolation, identification and degradation characteristics of a deltamethrin-degrading bacterial strain[J]. Freshwater Fisheries, 2019,49(1):98-106.
[5]	Zhao JY, Chi YL, et al. Effects of two surfactants and beta-cyclodex-trin on beta-cypermethrin degradation by Bacillus licheniformis B-1[J]. J Agric Food Chem, 2015,50:10729-10735.
[6]	Hao XX, Zhang XQ, Duan BH, et al. Screening and genome sequencing of deltamethrin-degrading bacterium ZJ6[J]. Curr Microbiol, 2018,75(11):1468-1476. URL pmid: 30066155
[7]	孙胜利. 焦化废水中产表面活性剂菌的筛选及其对多环芳烃降解的影响[D]. 广州:华南理工大学, 2018.
	Sun SL. The biosurfactant-producing microorganisms isolated from coking wastewater and its effect on the degradation of polycyclic aromatic hydrocarbons[D]. Guangzhou:South China University of Technology, 2018.
[8]	Zhang JS, Sun ZT, Li YY, et al. Biodegradation of p-nitrophenol by Rhodococcus sp. CN6 with high cell surface hydrophobicity[J]. Journal of Hazardous Materials, 2009,163(2-3):723-728.
[9]	Zhang C, Wang SH, Yan YC. Isomerization and biodegradation of beta-cypermethrin by Pseudomonas aeruginosa CH7 with biosurfactant production[J]. Bioresource Technology, 2011,102(14):7139-7146. URL pmid: 21570279
[10]	荣璐阁. 表面活性剂强化微生物修复柴油污染土壤研究[D]. 沈阳:沈阳大学, 2019.
	Rong LG. Study on microbial remediation of diesel contaminated soil by surfactant[D]. Shenyang:Shenyang University, 2019.
[11]	黄慧. 一株产生物表面活性剂的苯酚降解菌的筛选及性能研究[D]. 武汉:武汉科技大学, 2019.
	Huang H. Screening and characterization analysis of a phenol-degrading bacteria of producing biosurfactant[D]. Wuhan:Wuhan University of Science and Technology, 2019.
[12]	郭娟. 考马斯亮蓝G250与CTAB、OP-10的缔合作用研究[D]. 兰州:兰州交通大学, 2018.
	Guo J. The association interactions between CTAB、OP-10 and coomassie brilliant blue G250[D]. Lanzhou:Lanzhou Jiaotong University, 2018.
[13]	柏青海. CTAB及碳纤维负载碘活化PMS降解染料的研究[D]. 杭州:浙江理工大学, 2017.
	Bo QH. Degradation of dyes based on CTAB and lodine supported carbon fibers to activate PMS[D]. Hangzhou:Zhejiang University of Science and Technology, 2017.
[14]	黄敏, 谭丽泉, 余梅, 等. 表面活性剂对白腐真菌降解石油的影响[J]. 石油炼制与化工, 2012,43(8):77-81.
	Huang M, Tan LQ, Yu M, et al. Effect of surfactants on petroleum degradation by white rot fungi[J]. Petroleum Processing and Petrochemicals, 2012,43(8):77-81.
[15]	Wei ZS, Hessler CM, George S, et al. Influence of nonionic surfactants on fungal and bacterial degradation of hexane[J]. CLEAN-Soil, Air, Water, 2016,44(7):1-8.
[16]	尚琼琼, 张秀霞, 李振伟, 等. 表面活性剂复配强化微生物修复水土体系中的菲[J]. 石油学报(石油加工), 2018,34(3):574-580.
	Shang QQ, Zhang XX, Li ZW, et al. Enhancing microorganism remediation of phenanthrene in water-soil system by mixed surfactants[J]. Acta Petrolei Sinica(Petroleum Processing Section), 2018,34(3):574-580.
[17]	林维佳. 生物表面活性剂对菌株GY2B和F14降解菲的影响机制研究[D]. 广州:华南理工大学, 2017.
	Lin WJ. Mechanism of the effect of biosurfactants on the biodegradation of phenanthrene by bacterial strain GY2B and F14[D]. Guangzhou:South China University of Technology, 2017.
[18]	肖鹏飞, 李玉文, Kondo R. Tween60和SDS强化白腐真菌修复DDT污染土壤[J]. 中国环境科学, 2015,35(12):3737-3743.
	Xiao PF, Li YW, Kondo R. Enhancement of Tween60 and SDS for bioremediation of DDT-contaminated soil by white rot fungus[J]. China Environmental Science, 2015,35(12):3737-3743.
[19]	姜霞, 区自清, 应佩峰, 等. 表面活性剂对分枝杆菌KR2菌株降解菲的影响[J]. 应用生态学报, 2003(11):2011-2014.
	Jiang X, Qu ZQ, Ying PF, et al. Effects of surfactants on degradation of phenanthrene by Mycobacterium sp. KR2[J]. Chinese Journal of Applied Ecology, 2003(11):2011-2014.
[20]	Zhong H, Wang ZQ, Liu ZF, et al. Degradation of hexadecane by pseudomonas aeruginosa with the mediation of surfactants:Relation between hexadecane solubilization and bioavailability[J]. International Biodeterioration & Biodegradation, 2016,115:141-145.
[21]	王为中, 王向阳, 孙小涵, 等. 表面活性剂液相微萃取在白蚁药物残留分析中的应用研究[J]. 中华卫生杀虫药械, 2017(2):75-78.
	Wang WZ, Wang XY, Sun XH, et al. Application of surfactant-assisted dispersive liquid-phase micro-extraction for detection of termiticide residues[J]. Chinese Journal of Hygienic Insecticides & Equipments, 2017(2):75-78.
[22]	Ahmad S, Yasin K. Removal of organic pollutants by using surfactant modified bentonite[J]. Journal of the Chemical Society of Pakistan, 2018,3:447-456.
[23]	Cheng M, Zeng GM, Huang DL, et al. Tween 80 surfactant-enhanced bioremediation:toward a solution to the soil contamination by hydrophobic organic compounds[J]. Critical Reviews in Biotechnology, 2018,38(1):1-14. doi: 10.1080/07388551.2017.1311295 URL pmid: 28462596
[24]	龚铃堰, 廖广志, 陈权生, 等. 表面活性剂溶胀胶束:性能及应用[J]. 物理化学学报, 2019,35(8):816-828.
	Gong LY, Liao GZ, Chen QS, et al. Surfactant swelling micelles:properties and applications[J]. Acta Physico-Chimica Sinica, 2019,35(8):816-828.
[25]	胡琼, 唐洁, 雷丹, 等. 一株溴氰菊酯降解菌的分离鉴定及其降解条件优化[J]. 微生物学通报, 2020,47(3):699-709.
	Hu Q, Tang J, Lei D, et al. Isolation, identification and optimization of degradation conditions of a deltamethrin degrading strain[J]. Microbiology China, 2020,47(3):699-709.
[26]	张春燕, 罗建新, 潘春跃, 等. 光谱法测定十二烷基苯磺酸钠临界胶束浓度[J]. 环境科学与技术, 2016,39(8):99-102.
	Zhang CY, Luo JX, Pang CY, et al. Determination of critical micelle concentration of sodium dodecyl benzene sulfonate by spectrometry[J]. Environ Sci Technol, 2016,39(8):99-102.
[27]	王冠石, 王小永. 添加中低浓度离子液体[bmim][BF₄]对吐温-20临界胶束浓度和胶束结构的影响[J]. 化学通报, 2017,80(8):777-782.
	Wang GS, Wang XY. Influence of medium-low concentration of ionic liquid[Bmim][BF₄]on critical micellization concentration and micelle structures[J]. Chemistry, 2017,80(8):777-782.
[28]	张栋. 表面活性剂对PAHs微生物界面行为的影响及调控机制[D]. 杭州:浙江大学, 2013.
	Zhang D. Surfactant controlled bacterial interfacial behaviors of PAHs and its mechanisms[D]. Hangzhou:Zhejiang University, 2013.
[29]	鲍根莲, 邓欢欢, 李少君, 等. 生物表面活性剂和化学螯合剂强化无柄小叶榕修复Cd、Cu重金属污染盐碱地[J]. 环境化学, 2019,38(7):1497-1506.
	Bao GL, Deng HH, LI SJ, et al. Biosurfactants generated from marine microbe and chelator assisted remediation of Cadmium and Copper contaminated saline-alkaline soil by Ficus concinna var. Subsessilis[J]. Environ Chem, 2019,38(7):1497-1506.
[30]	Liu S, Guo C, Liang X, et al. Nonionic surfactants induced changes in cell characteristics and phenanthrene degradation ability of Sphingomonas sp. GY2B[J]. Ecotoxicology and Environmental Safety, 2016,129:210-218. URL pmid: 27045921
[31]	Tehrani-Bagha AR, Holmberg K. Solubilization of hydrophobic dyes in surfactant solutions[J]. Materials, 2013,6(2):580-608. doi: 10.3390/ma6020580 URL pmid: 28809328
[32]	Lee SY, Kim SJ, Chung SY, et al. Sorption of hydrophobic organic compounds onto organoclays[J]. Chemosphere, 2004,55(5):781-785. doi: 10.1016/j.chemosphere.2003.11.007 URL pmid: 15013684
[33]	González M, Ma DM, Sánchez L, et al. Pesticide adsorption on a calcareous soil modified with sewage sludge and quaternary alkyl-ammonium cationic surfactants[J]. Environmental Science & Pollution Research, 2008,15(1):8-14. doi: 10.1065/espr2007.02.387 URL pmid: 18306881
[34]	Lu L, Zhu L. Effect of a cationic surfactant on the volatilization of PAHs from soil[J]. Environmental Science & Pollution Research International, 2012,19(5):1515-1523. doi: 10.1007/s11356-011-0631-0 URL pmid: 21997282
[35]	闫端, 余晖, 黄国和, 等. 双子表面活性剂CG_12-3-12、鼠李糖脂与TX-100对多环芳烃增溶作用的比较研究[J]. 环境科学学报, 2015,35(1):229-237.
	Yan D, Yu H, Huang GH, et al. Effects of gemini surfactant CG_12-3-12,rhamnolipid and triton- X-100 on solubility enhancement of PAHs:A comparative study[J]. Acta Scientiae Circumstantiae, 2015,35(1):229-237.
[36]	Zhang H, Zhang YM, Hou ZG, et al. Biodegradation potential of deltamethrin by the Bacillus cereus strain Y1 in both culture and contaminated soil[J]. Int Biodeter Biodegradation, 2016,106:53-59.
[37]	侯少锋. 溴氰菊酯降解菌剂的制备与应用[D]. 贵阳:贵州大学, 2016.
	Hou SF. Preparation and application of deltamethrin degrading bacteria agent[D]. Guiyang:Guizhou University, 2016.
[38]	杨晓棠. 溴氰菊酯降解菌的筛选及降解特性研究[D]. 长春:吉林农业大学, 2008.
	Yang XT. Study on isolation and degrading characteristics of deltamethrin-degrading bacteria[D]. Changchun:Jilin Agricultural University, 2008.
[39]	向骊羽, 赵浩宇, 黄孟飞, 等. 一株溴氰菊酯降解菌的筛选及其降解特性研究[J]. 四川大学学报:自然科学版, 2012,49(4):914-918.
	Xiang LY, Zhao HY, Huang MF, et al. Isolation and identification of a strain of deltamethrin degrading bacteria and its degradation characteristics[J]. Journal of Sichuan University:Natural Science Edition, 2012,49(4):914-918.