脂质组学在毒理学研究中的应用

doi:10.13560/j.cnki.biotech.bull.1985.2022-0793

摘要/Abstract

摘要：

脂质作为细胞的主要组成部分，在细胞内物质运输、信号转导、细胞凋亡等多种生命活动中发挥重要作用。脂质组学通过对生物体内的脂质进行系统分析，从而了解其相互作用以及与其他生物分子之间的作用。主要介绍了脂质组学的研究方法，包括样品前处理方法、检测方法和仪器设备以及数据分析方法。同时，综述了脂质组学技术在神经毒性、肝脏毒性、免疫毒性以及内分泌干扰毒性中的研究进展，以期为研究化合物毒性作用机制提供思路和参考。最后，提出未来应利用脂质组学技术开展不同化合物的联合毒性作用研究，通过脂质组学筛选获得相关脂质生物标志物，为联合毒性作用的风险预警和相关毒性作用机制分析提供科学依据。

关键词: 脂质, 脂质组学, 毒理学, 分析方法

Abstract:

As major components of cells, lipids play important roles in a variety of life activities, such as intracellular material transport, signal transduction and apoptosis, etc. Lipidomics provides a systematic analysis of lipids in living organisms to understand their interactions and the interactions with other biomolecules. The authors mainly introduced the analysis methods of lipidomics, including the pretreatment methods of samples, detection methods and instruments, and data analysis methods. Besides that, the authors reviewed the research progress of lipidomics in neurotoxicity, hepatotoxicity, immunotoxicity and endocrine disrupting toxicity, aiming to provide ideas and reference for studying the mechanism of compound toxicity. Finally, the authors suggested that lipidomics technology should be used to study the combined toxic effects of different compounds in the future, to screen relevant lipid biomarkers based on the lipidomics, as well as to provide the scientific basis for risk warning of combined toxicity and relevant toxic mechanism study.

Key words: lipids, lipidomics, toxicology, analysis methods

王昕璐, 王蒙, 翟文磊. 脂质组学在毒理学研究中的应用[J]. 生物技术通报, 2023, 39(3): 69-80.

WANG Xin-lu, WANG Meng, ZHAI Wen-lei. Application of Lipidomics in Toxicological Studies[J]. Biotechnology Bulletin, 2023, 39(3): 69-80.

图/表 4

参考文献 77

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序号 No.	样品类别 Sample type	提取方式 Extraction method	分析仪器 Analysis instrument	毒性研究目标物 Toxicity research target	参考文献 Reference
1	斑马鱼肝细胞	氯仿/甲醇（2:1，V/V）+0.01%丁羟甲苯	UHPLC-Q Exactive-MS	双酚A、双酚F、双酚3-氯-2-羟丙基醚	[42]
2	C57BL/6J鼠	甲基叔丁基醚/甲醇/水（10:3:3，V/V/V）	LC-MS/MS	多溴联苯醚	[43]
3	雄性昆明鼠血清	十七烷酸/甲醇（7:2，V/V）	GC-MS	万古霉素	[44]
4	太平洋白虾	氯仿-甲醇体系	LC-MS/MS	微囊藻素	[45]
5	PC12细胞	二氯甲烷/甲醇（1:2，V/V）	UHPLC-MS/MS	多氯联苯（PCB153）	[29]
6	雌性ICR小鼠	氯仿/甲醇/水（3:4:1，V/V/V）	AP-MALDI-MS	镉	[46]
7	斑马鱼胚胎	氯仿/甲醇/水（2:1:1，V/V/V）	LC-QTOF-MS	全氟己烷磺酸	[47]
8	小鼠大脑皮层	甲醇/甲基叔丁基醚（3:10，V/V）	UHPLC-Q Exactive plus-MS	雄黄	[48]
9	小鼠脑组织	甲醇/甲基叔丁基醚（3:10，V/V）	LC-MS/MS	多溴联苯醚（BDE-47）	[49]
10	小鼠脑组织、血液、粪便	甲醇/甲基叔丁基醚（1:5，V/V）	UHPLC-Q Exactive Focus-MS	砷	[50]
11	猕猴血清、脑组织	氯仿/甲醇/水（1:2:0.8，V/V/V）	LC-MS/MS	七氟烷	[51]
12	Neuro-2a细胞	二氯甲烷/甲醇（1:2，V/V）	UHPLC-Q Exactive plus-MS	吡虫啉、啶虫脒	[12]
13	小鼠肝脏组织	氯仿/甲醇（2:1，V/V）	LC-MS/MS	邻苯二甲酸酯	[52]
14	人肝细胞	甲醇/甲基叔丁基醚	UHPLC-Q Exactive-MS	二甲基甲酰胺	[53]
15	HepG2细胞	甲醇/甲基叔丁基醚（1:3，V/V）	LC-QTOF-MS	聚乙烯微塑料、多氯联苯	[54]
16	小鼠肝脏组织	甲醇	UPLC-ESI-Orbitrap-MS	全氟己烷磺酸	[55]

序号 No.	样品类别 Sample type	提取方式 Extraction method	分析仪器 Analysis instrument	毒性研究目标物 Toxicity research target	参考文献 Reference
1	斑马鱼肝细胞	氯仿/甲醇（2:1，V/V）+0.01%丁羟甲苯	UHPLC-Q Exactive-MS	双酚A、双酚F、双酚3-氯-2-羟丙基醚	[42]
2	C57BL/6J鼠	甲基叔丁基醚/甲醇/水（10:3:3，V/V/V）	LC-MS/MS	多溴联苯醚	[43]
3	雄性昆明鼠血清	十七烷酸/甲醇（7:2，V/V）	GC-MS	万古霉素	[44]
4	太平洋白虾	氯仿-甲醇体系	LC-MS/MS	微囊藻素	[45]
5	PC12细胞	二氯甲烷/甲醇（1:2，V/V）	UHPLC-MS/MS	多氯联苯（PCB153）	[29]
6	雌性ICR小鼠	氯仿/甲醇/水（3:4:1，V/V/V）	AP-MALDI-MS	镉	[46]
7	斑马鱼胚胎	氯仿/甲醇/水（2:1:1，V/V/V）	LC-QTOF-MS	全氟己烷磺酸	[47]
8	小鼠大脑皮层	甲醇/甲基叔丁基醚（3:10，V/V）	UHPLC-Q Exactive plus-MS	雄黄	[48]
9	小鼠脑组织	甲醇/甲基叔丁基醚（3:10，V/V）	LC-MS/MS	多溴联苯醚（BDE-47）	[49]
10	小鼠脑组织、血液、粪便	甲醇/甲基叔丁基醚（1:5，V/V）	UHPLC-Q Exactive Focus-MS	砷	[50]
11	猕猴血清、脑组织	氯仿/甲醇/水（1:2:0.8，V/V/V）	LC-MS/MS	七氟烷	[51]
12	Neuro-2a细胞	二氯甲烷/甲醇（1:2，V/V）	UHPLC-Q Exactive plus-MS	吡虫啉、啶虫脒	[12]
13	小鼠肝脏组织	氯仿/甲醇（2:1，V/V）	LC-MS/MS	邻苯二甲酸酯	[52]
14	人肝细胞	甲醇/甲基叔丁基醚	UHPLC-Q Exactive-MS	二甲基甲酰胺	[53]
15	HepG2细胞	甲醇/甲基叔丁基醚（1:3，V/V）	LC-QTOF-MS	聚乙烯微塑料、多氯联苯	[54]
16	小鼠肝脏组织	甲醇	UPLC-ESI-Orbitrap-MS	全氟己烷磺酸	[55]